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In biochemistry and molecular biology, the saline-sodium citrate (SSC) buffer is used as a hybridization buffer, to control stringency for washing steps in protocols for Southern blotting, in situ hybridization, DNA Microarray or Northern blotting. 20X SSC may be used to prevent drying of agarose gels during a vacuum transfer.
- Material safety data sheet for 20x SSC buffer, Mediatech, Inc.
- Molecular Cytogenetics and biology Buffers, University of Leicester
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