# STED microscopy

Stimulated emission depletion (STED) microscopy is a process that provides super resolution by selectively deactivating fluorophores to enhance the imaging in that area.[1] It was developed by Stefan W. Hell in 1994,[2] and was first experimentally shown in 1999. This is one of several types of super resolution microscopy techniques that have recently been developed. Super resolution microscopy is a set of techniques to bypass the diffraction limit of microscopy to achieve better resolution. Both photoactivated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM) are also super resolution microscopy techniques, although they use a different process than STED to achieve this resolution.

## Background

Jablonski diagram showing the redshift of the stimulated photon. This redshift allows the stimulated photon to be ignored.
Diagram of the design of a STED device. The double laser design allows for excitation and stimulated emission to be used together for STED.

In traditional microscopy, the resolution that can be obtained is limited by the diffraction of light. Ernst Abbe developed an equation to describe this limit. The equation is:

$\mathrm{D} = \frac{\lambda}{\mathrm{NA}}$

where D is the diffraction limit, λ is the wavelength of the light, and NA is the numerical aperture, or the refractive index of the medium multiplied by the sine of the angle of incidence. This diffraction limit is the standard by which all super resolution methods are measured. Because STED selectively deactivates the fluorescence, it can achieve resolution better than the traditional confocal microscopy. Normal fluorescence occurs by exciting an electron from the ground state into an excited electronic state which, after relaxing back to the ground state, emits a photon. STED interrupts this process before the photon is released. The excited electron is forced to relax into a higher vibration state than the fluorescence transition would enter, causing the photon to be released to be red shifted as shown in the image below.[3] Because the electron is going to a higher vibrational state, the energy difference of the two states is lower than the normal fluorescence difference. This lowering of energy raises the wavelength, and causes the photon to be shifted farther into the red end of the spectrum. This shift differentiates the two types of photons, and allows the stimulated photon to be ignored.

To force this alternative emission to occur, an incident photon must strike the fluorophore. This need to be struck by an incident photon has two implications for STED. First, the number of incident photons directly impacts the efficiency of this emission, and, secondly, with sufficiently large numbers of photons fluorescence can be completely suppressed.[4] To achieve the large number of incident photons needed to suppress fluorescence, the laser used to generate the photons must be of a high intensity. Unfortunately, this high intensity laser can lead to the issue of photobleaching the fluorophore. Photobleaching is the name for the destruction of fluorophores by high intensity light.

## Process

Comparison of confocal microscopy and STED microscopy. This shows the improved resolution of STED microscopy over traditional techniques.
Excitation spot (2D, left), doughnut-shape de-excitation spot (center) and remaining area allowing fluorescence (right).

STED functions by depleting specific regions of the sample while leaving a center focal spot active to emit fluorescence. This focal area can be engineered by altering the properties of the pupil plane of the objective lens.[5][6][7] The most common early example of this diffractive optical elements, or DOEs, is a doughnut shape used in two dimensional lateral confinement shown below. The red zone is depleted, while the green spot is left active. This DOE is generated by a circular polarization of the depletion laser, combined with a helical phase ramp. The lateral resolution of this DOE is typically between 30 and 80 nm. However, values down to 2.4 nm have been reported.[8] Using different DOEs, axial resolution on the order of 100 nm have been demonstrated.[9] A modified Abbe’s equation describes this sub diffraction resolution as:

$\mathrm{D} = \frac{\lambda}{2 n \sin{\alpha} * \sqrt{1 + \frac{I}{Isat}}}$

To optimize the effectiveness of STED, the destructive interference in the center of the focal spot needs to be as close to perfect as possible. That imposes certain constraints on the optics that can be used.

## Dyes

Early on in the development of STED, the number of dyes that could be used in the process was very limited. Rhodamine B was named in the first theoretical description of STED.[2] As a result, the first dyes used were laser emitting in the red spectrum. To allow for STED analysis of biological systems, the dyes and laser sources must be tailored to the system. This desire for better analysis of these systems has led to living cell STED and multicolor STED, but it has also demanded more and more advanced dyes and excitation systems to accommodate the increased functionality.[3]

One such advancement was the development of immunolabeled cells. These cells are STED fluorescent dyes bound to antibodies through amide bonds. The first use of this technique coupled MR-121SE, a red dye, with a secondary anti-mouse antibody.[4] Since that first application, this technique has been applied to a much wider range of dyes including green emitting, Atto 532,[10][11][12] and yellow emitting, Atto 590,[13] as well as additional red emitting dyes. In addition, Atto 647N was first used with this method to produce two-color STED.[14]

## Applications

Over the last several years, STED has developed from a complex and highly specific technique to a general fluorescence method. As a result, a number of methods have been developed to expand the utility of STED and to allow more information to be provided.

### Structural analysis

From the beginning of the process, STED has allowed fluorescence microscopy to perform tasks that had been only possible using electron microscopy. For example, STED was used for the elucidation of protein structure analysis at sub-organelle level. The common proof of this level of study is the observation of cytoskeletal filaments. In addition, neurofilaments, actin, and tubulin are often used to compare the resolving power of STED and confocal microscopes.[15][16][17]

Using STED, a lateral resolution of 70 – 90 nm has been achieved while examining SNAP25, a human protein that regulates membrane fusion. This observation has shown that SNAP25 forms clusters independently of the SNARE motif’s functionality, and binds to clustered syntaxin.[18][19] Studies of complex organelles, like mitochondria, also benefit from STED microscopy for structural analysis. Using custom-made STED microscopes with a lateral resolution of fewer than 50 nm, mitochondrial proteins Tom20, VDAC1, and COX2 were found to distribute as nanoscale clusters.[20][21]

### Correlative methods

Due to its function, STED microscopy can often be used with other high-resolution methods. The resolution of both electron and atomic force microscopy is even better than STED resolution, but by combining atomic force with STED, Shima et al. was able to visualize the actin cytoskeleton of human ovarian cancer cells while observing changes in cell stiffness.[22]

### Multicolor

Multicolor STED was developed in response to a growing problem in using STED to study the dependency between structure and function in proteins. To study this type of complex system, at least two separate fluorophores must be used. Using two fluorescent dyes and beam pairs, colocalized imaging of synaptic and mitochondrial protein clusters is possible with a resolution down to 5 nm [18]. Multicolor STED has also been used to show that different populations of synaptic vesicle proteins do not mix of escape synaptic boutons.[23][24] By using two color STED with multi-lifetime imaging, three channel STED is possible.

### Live-cell

Early on, STED was thought to be a useful technique for working with living cells.[9] Unfortunately, the only way for cells to be studied was to label the plasma membrane with organic dyes.[23] Combining STED with fluorescence correlation spectroscopy showed that cholesterol-mediated molecular complexes trap sphingolipids, but only transiently.[25] However, only fluorescent proteins provide the ability to visualize any organelle or protein in a living cell. This method was shown to work at 50 nm lateral resolution within Citrine-tubulin expressing mammal cells.[26][27] In addition to detecting structures in mammal cells, STED has allowed for the visualization of clustering YFP tagged PIN proteins in the plasma membrane of plant cells.[28]

Recently, multicolor live-cell STED was performed using a pulsed far-red laser and CLIPf-tag and SNAPf-tag expression.[29]

### STED at video rates

Super-resolution requires small pixels, which means more spaces to acquire from in a given sample, which leads to a longer acquisition time. However, the focal spot size is dependent on the intensity of the laser being used for depletion. As a result, this spot size can be tuned, changing the size and imaging speed. A compromise can then be reached between these two factors for each specific imaging task. Rates of 80 frames per second have been recorded, with focal spots around 60 nm.[1][30]

## Problems

Photobleaching can occur either from excitation into an even higher excited state, or from excitation in the triplet state. To prevent the excitation of an excited electron into another, higher excited state, the energy of the photon needed to trigger the alternative emission should not overlap the energy of the excitation from one excited state to another.[31] This will ensure that each laser photon that contacts the fluorophores will cause stimulated emission, and not cause the electron to be excited to another, higher energy state. Triplet states are much longer lived than singlet states, and to prevent triplet states from exciting, the time between laser pulses needs to be long enough to allow the electron to relax through another quenching method, or a chemical compound should be added to quench the triplet state.[15][32][33]

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