STX8

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Syntaxin 8
Protein STX8 PDB 1gl2.png
PDB rendering based on 1gl2.
Available structures
PDB Ortholog search: PDBe, RCSB
Identifiers
Symbols STX8 ; CARB
External IDs OMIM604203 MGI1890156 HomoloGene37973 GeneCards: STX8 Gene
RNA expression pattern
PBB GE STX8 204690 at tn.png
PBB GE STX8 gnf1h09415 s at tn.png
PBB GE STX8 gnf1h09416 s at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez 9482 55943
Ensembl ENSG00000170310 ENSMUSG00000020903
UniProt Q9UNK0 O88983
RefSeq (mRNA) NM_004853 NM_018768
RefSeq (protein) NP_004844 NP_061238
Location (UCSC) Chr 17:
9.15 – 9.48 Mb
Chr 11:
67.97 – 68.21 Mb
PubMed search [1] [2]

Syntaxin-8 is a protein that in humans is encoded by the STX8 gene.[1][2][3] Syntaxin 8 directly interacts with HECTd3 and has similar subcellular localization.[4] The protein has been shown to form the SNARE complex with syntaxin 7, vti1b and endobrevin. These function as the machinery for the homotypic fusion of late endosomes.[5]

Model organisms[edit]

Model organisms have been used in the study of STX8 function. A conditional knockout mouse line, called Stx8tm2a(EUCOMM)Wtsi[10][11] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists — at the Wellcome Trust Sanger Institute.[12][13][14] Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[8][15] Twenty four tests were carried out on homozygous mutant adult mice, however no significant abnormalities were observed.[8]

Interactions[edit]

STX8 has been shown to interact with Vesicle-associated membrane protein 8,[16] VTI1B[17][18] and STX7.[16]

References[edit]

  1. ^ Steegmaier M, Yang B, Yoo JS, Huang B, Shen M, Yu S, Luo Y, Scheller RH (January 1999). "Three novel proteins of the syntaxin/SNAP-25 family". J Biol Chem 273 (51): 34171–9. doi:10.1074/jbc.273.51.34171. PMID 9852078. 
  2. ^ Thoreau V, Berges T, Callebaut I, Guillier-Gencik Z, Gressin L, Bernheim A, Karst F, Mornon JP, Kitzis A, Chomel JC (May 1999). "Molecular cloning, expression analysis, and chromosomal localization of human syntaxin 8 (STX8)". Biochem Biophys Res Commun 257 (2): 577–83. doi:10.1006/bbrc.1999.0503. PMID 10198254. 
  3. ^ "Entrez Gene: STX8 syntaxin 8". 
  4. ^ Zhang L, Kang L, Bond W, Zhang N (February 2009). "Interaction between syntaxin 8 and HECTd3, a HECT domain ligase". Cell Mol Neurobiol 29 (1): 115–21. doi:10.1007/s10571-008-9303-0. PMID 18821010. 
  5. ^ Kasai K, Suga K, Izumi T, Akagawa K (October 2008). "Syntaxin 8 has two functionally distinct di-leucine-based motifs". Cell Mol Bio Lett 13 (1): 144–54. doi:10.2478/s11658-007-0043-9. PMID 17965969. 
  6. ^ "Salmonella infection data for Stx8". Wellcome Trust Sanger Institute. 
  7. ^ "Citrobacter infection data for Stx8". Wellcome Trust Sanger Institute. 
  8. ^ a b c Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica 88 (S248). doi:10.1111/j.1755-3768.2010.4142.x. 
  9. ^ Mouse Resources Portal, Wellcome Trust Sanger Institute.
  10. ^ "International Knockout Mouse Consortium". 
  11. ^ "Mouse Genome Informatics". 
  12. ^ Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.  edit
  13. ^ Dolgin E (June 2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718. 
  14. ^ Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. 
  15. ^ van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism.". Genome Biol 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353. 
  16. ^ a b Antonin, W; Holroyd C, Fasshauer D, Pabst S, Von Mollard G F, Jahn R (December 2000). "A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function". EMBO J. (ENGLAND) 19 (23): 6453–64. doi:10.1093/emboj/19.23.6453. ISSN 0261-4189. PMC 305878. PMID 11101518. 
  17. ^ Rual, Jean-François; Venkatesan Kavitha, Hao Tong, Hirozane-Kishikawa Tomoko, Dricot Amélie, Li Ning, Berriz Gabriel F, Gibbons Francis D, Dreze Matija, Ayivi-Guedehoussou Nono, Klitgord Niels, Simon Christophe, Boxem Mike, Milstein Stuart, Rosenberg Jennifer, Goldberg Debra S, Zhang Lan V, Wong Sharyl L, Franklin Giovanni, Li Siming, Albala Joanna S, Lim Janghoo, Fraughton Carlene, Llamosas Estelle, Cevik Sebiha, Bex Camille, Lamesch Philippe, Sikorski Robert S, Vandenhaute Jean, Zoghbi Huda Y, Smolyar Alex, Bosak Stephanie, Sequerra Reynaldo, Doucette-Stamm Lynn, Cusick Michael E, Hill David E, Roth Frederick P, Vidal Marc (October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature (England) 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. 
  18. ^ Subramaniam, V N; Loh E, Horstmann H, Habermann A, Xu Y, Coe J, Griffiths G, Hong W (March 2000). "Preferential association of syntaxin 8 with the early endosome". J. Cell. Sci. (ENGLAND) 113 (6): 997–1008. ISSN 0021-9533. PMID 10683148. 

Further reading[edit]