Lord Mitsuyoshi/Yagyū Jūbei - the protagonist. He is the master of "Yagyu Shinkagery" sword style and the one responsible for guarding the Yagyu Secret Chronicles.
Lord Gomino - the main antagonist and the former emperor. He resides in Kyoto and attempts to start a rebellion against the Tokugawa bakufu which is the reason as to why he sent his lackey Yashamaro to steal the Yagyu Secret Chronicles.
Lord Rokumaru - Jubei's younger half-brother and chief monk of the Hotokuji Temple. He aides his brother in retrieving the Yagyu Secret Chronicles.
Yashamaro - Gomino's ninja whom he sent to steal the Yagyu Secret Chronicles. He is Jubei's foe.
Yagyū Munenori - Jubei's father and katana instructor for the Shogun and watcher of all feudal lords. He is also the leader of the secret organization of Shadow Yagyu.
Lady Tsukinowanomiya called Lady Miya - Gomino's daughter and granddaughter of Tokugawa Hidetada. She was once the Empress Myosho but retired. She is an old friend of Jubei.