Sequence of proteases – chymotrypsin A – trypsin – elastase

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(Serpin)-The X-ray crystal structure of the archetypal serine protease chymotrypsin (pdb code 4CHA). The three catalytic residues (His 57, Asp 102 and Ser 195) are labeled.

In the catalytic triad of chymotrypsin, trypsin, and elastase, the three proteases function at three important molecular 'cutting' points. The sites are at amino acid residues of: histidine 57, aspartic acid 102, and serine 195.

The comparison for chymotrypsin A (cow), and trypsin (cow), and elastase (pig) is as follows:[1]

Site: aspartic acid 102[edit]

Section showing aspartic acid 102:[2]

-

x 73 75 80 85 90 95 99A
C QGSSSEKI QKLKI AKVFK NSKYN SLTI'
T INVVEGNQ QFISA SKSIV HPSYN SNTL'
E LNQNNGTE QYVGV QKIVV HPYWN TDDVA

- (D=Aspartic Acid 102)

x 99B 102,3,4,5 106-110 115 120 121 5,126
C 'NNDITL LKLST AASFS QTVSA VCLPSA
T 'NNDITL IKLKS AASLN SRVAS ISLPT'
E AGYDIAL LRLAQ SVTLN SYVQL GVLPRA

-

Entire comparison of the sequences[edit]

-

x 1,2,3,4,5 6-10 15 20
C-Chymotrypsin CGVPA IQPVL SGL(SR) IVNGE
T-Trypsin '`'`' `'`'(V DDDDK) IVGGY
E-Elastase '`'`' `'`'` '`'`' VVGGT

-

x 30 35 36ABC37 40 45
C EAPVG SWPWQ DK'`'TGTH FCGGS
T TCGAN TVPYQ '`'`'SGYH FCGGS
E EAQRN SWPSQ ISLQYRSGSWAH TCGGT

- Section showing histidine 57:[3] -

x 46-50 55 56,57-60 65 65A 66-70
C LINEN WVVTA AHCGV TTSDV 'VVAGEFD
T LINSQ WVVSA AHCYK SGIQV RL'`GQDN
E LIRQN WVMTA AHCVD RELTF RVVVGEHN

-

x 73-75-80 85 90 95 96-99A
C QGSSSEKI QKLKI AKVFK NSKYN SLTI'
T INVVEGNQ QFISA SKSIV HPSYN SNTL'
E LNQNNGTE QYVGV QKIVV HPYWN TDDVA

-

x 99B 102,3,4,5 106-110 115 120 121 5,126
C 'NNDITL LKLST AASFS QTVSA VCLPSA
T 'NNDITL IKLKS AASLN SRVAS ISLPT'
E AGYDIAL LRLAQ SVTLN SYVQL GVLPRA

-

Remaining half of the sequence, 127-245, not shown. (site serine 195, not shown)

See also[edit]

References[edit]

  1. ^ Wilson, Eisner, Briggs, Dickerson, Metzenberg, O'Brien, Susman, & Boggs. Life on Earth, Chapter: Molecular Evolution, Graphic: Sequences of Four Proteases, (cow and pig, etc.) p. 816-817.
  2. ^ Life on Earth, Graphic: Sequences of Four Proteases, (cow and pig, etc.) p. 816-817.
  3. ^ Life on Earth, Graphic: Sequences of Four Proteases, (cow and pig, etc.) p. 816-817.
  • Wilson, Eisner, Briggs, Dickerson, Metzenberg, O'Brien, Susman, & Boggs. Life on Earth, Edward O. Wilson, Thomas Eisner, Winslow R. Briggs, Richard E. Dickerson, Robert L. Metzenberg, Richard D. O'Brien, Millard Susman, William E. Boggs, c 1973, Sinauer Associates, Inc., Publisher, Stamford, Connecticut. 1033 pp, 19 p Index & Back Page (hardcover, ISBN 0-87893-934-2)