Sequestosome 1

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Sequestosome 1
Protein SQSTM1 PDB 1q02.png
PDB rendering based on 1q02.
Available structures
PDB Ortholog search: PDBe, RCSB
Symbols SQSTM1 ; A170; OSIL; PDB3; ZIP3; p60; p62; p62B
External IDs OMIM601530 MGI107931 HomoloGene31202 GeneCards: SQSTM1 Gene
RNA expression pattern
PBB GE SQSTM1 201471 s at tn.png
PBB GE SQSTM1 213112 s at tn.png
More reference expression data
Species Human Mouse
Entrez 8878 18412
Ensembl ENSG00000161011 ENSMUSG00000015837
UniProt Q13501 Q64337
RefSeq (mRNA) NM_001142298 NM_001290769
RefSeq (protein) NP_001135770 NP_001277698
Location (UCSC) Chr 5:
179.23 – 179.27 Mb
Chr 11:
50.2 – 50.21 Mb
PubMed search [1] [2]

Sequestosome-1 is a protein that in humans is encoded by the SQSTM1 gene.[1][2][3]

Model organisms[edit]

Model organisms have been used in the study of SQSTM1 function. A conditional knockout mouse line, called Sqstm1tm1a(KOMP)Wtsi[9][10] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.[11][12][13]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[7][14] Twenty two tests were carried out on homozygous mutant mice and one significant abnormality was observed: females had abnormal complete blood count parameters, including an increased red blood cell distribution width and increased mean platelet volume.[7]


Sequestosome 1 has been shown to interact with RAD23A,[15] TrkA,[16][17][18][19] TrkB,[17][18] PRKCI,[20] RIPK1,[21] TRAF6[22] and MAP1LC3A.[23]


  1. ^ Joung I, Strominger JL, Shin J (July 1996). "Molecular cloning of a phosphotyrosine-independent ligand of the p56lck SH2 domain". Proc Natl Acad Sci U S A 93 (12): 5991–5. doi:10.1073/pnas.93.12.5991. PMC 39176. PMID 8650207. 
  2. ^ Devergne O, Hummel M, Koeppen H, Le Beau MM, Nathanson EC, Kieff E, Birkenbach M (February 1996). "A novel interleukin-12 p40-related protein induced by latent Epstein-Barr virus infection in B lymphocytes". J Virol 70 (2): 1143–53. PMC 189923. PMID 8551575. 
  3. ^ "Entrez Gene: SQSTM1 sequestosome 1". 
  4. ^ "Haematology data for Sqstm1". Wellcome Trust Sanger Institute. 
  5. ^ "Salmonella infection data for Sqstm1". Wellcome Trust Sanger Institute. 
  6. ^ "Citrobacter infection data for Sqstm1". Wellcome Trust Sanger Institute. 
  7. ^ a b c Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. 
  8. ^ Mouse Resources Portal, Wellcome Trust Sanger Institute.
  9. ^ "International Knockout Mouse Consortium". 
  10. ^ "Mouse Genome Informatics". 
  11. ^ Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.  edit
  12. ^ Dolgin E (2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718. 
  13. ^ Collins FS, Rossant J, Wurst W (2007). "A Mouse for All Reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. 
  14. ^ van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism.". Genome Biol 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353. 
  15. ^ Rual, Jean-François; Venkatesan Kavitha, Hao Tong, Hirozane-Kishikawa Tomoko, Dricot Amélie, Li Ning, Berriz Gabriel F, Gibbons Francis D, Dreze Matija, Ayivi-Guedehoussou Nono, Klitgord Niels, Simon Christophe, Boxem Mike, Milstein Stuart, Rosenberg Jennifer, Goldberg Debra S, Zhang Lan V, Wong Sharyl L, Franklin Giovanni, Li Siming, Albala Joanna S, Lim Janghoo, Fraughton Carlene, Llamosas Estelle, Cevik Sebiha, Bex Camille, Lamesch Philippe, Sikorski Robert S, Vandenhaute Jean, Zoghbi Huda Y, Smolyar Alex, Bosak Stephanie, Sequerra Reynaldo, Doucette-Stamm Lynn, Cusick Michael E, Hill David E, Roth Frederick P, Vidal Marc (October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature (England) 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. 
  16. ^ Wooten, M W; Seibenhener M L; Mamidipudi V; Diaz-Meco M T; Barker P A; Moscat J (March 2001). "The atypical protein kinase C-interacting protein p62 is a scaffold for NF-kappaB activation by nerve growth factor". J. Biol. Chem. (United States) 276 (11): 7709–12. doi:10.1074/jbc.C000869200. ISSN 0021-9258. PMID 11244088. 
  17. ^ a b Geetha T, Wooten MW (2003). "Association of the atypical protein kinase C-interacting protein p62/ZIP with nerve growth factor receptor TrkA regulates receptor trafficking and Erk5 signaling.". J Biol Chem 278 (7): 4730–9. doi:10.1074/jbc.M208468200. PMID 12471037. 
  18. ^ a b Jadhav T, Geetha T, Jiang J, Wooten MW (2008). "Identification of a consensus site for TRAF6/p62 polyubiquitination.". Biochem Biophys Res Commun 371 (3): 521–4. doi:10.1016/j.bbrc.2008.04.138. PMC 2474794. PMID 18457658. 
  19. ^ Wooten MW, Geetha T, Babu JR, Seibenhener ML, Peng J, Cox N, Diaz-Meco MT, Moscat J (2008). "Essential role of sequestosome 1/p62 in regulating accumulation of Lys63-ubiquitinated proteins.". J Biol Chem 283 (11): 6783–9. doi:10.1074/jbc.M709496200. PMID 18174161. 
  20. ^ Sanchez, P; De Carcer G, Sandoval I V, Moscat J, Diaz-Meco M T (May 1998). "Localization of atypical protein kinase C isoforms into lysosome-targeted endosomes through interaction with p62". Mol. Cell. Biol. (UNITED STATES) 18 (5): 3069–80. ISSN 0270-7306. PMC 110686. PMID 9566925. 
  21. ^ Sanz L, Sanchez P, Lallena MJ, Diaz-Meco MT, Moscat J (1999). "The interaction of p62 with RIP links the atypical PKCs to NF-kappaB activation.". EMBO J 18 (11): 3044–53. doi:10.1093/emboj/18.11.3044. PMC 1171386. PMID 10356400. 
  22. ^ Sanz, L; Diaz-Meco M T; Nakano H; Moscat J (April 2000). "The atypical PKC-interacting protein p62 channels NF-kappaB activation by the IL-1-TRAF6 pathway". EMBO J. (ENGLAND) 19 (7): 1576–86. doi:10.1093/emboj/19.7.1576. ISSN 0261-4189. PMC 310227. PMID 10747026. 
  23. ^ Shvets E, Fass E, Scherz-Shouval R, Elazar Z (2008). "The N-terminus and Phe52 residue of LC3 recruit p62/SQSTM1 into autophagosomes.". J Cell Sci 121 (Pt 16): 2685–95. doi:10.1242/jcs.026005. PMID 18653543. 

Further reading[edit]