Sf9

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Sf9 cells, a clonal isolate of Spodoptera frugiperda Sf21 cells (IPLB-SF21-AE), are commonly used for recombinant protein production using baculovirus. They were originally established from ovarian tissue.[1] They can be grown in the absence of serum, and can be cultured attached or in suspension.[2]

References[edit]

  1. ^ Vaughn JL, Goodwin RH, Tompkins GJ, McCawley P (1977). "The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae)". In Vitro 13 (4): 213–217. doi:10.1007/BF02615077. PMID 68913. 
  2. ^ Invitrogen (03/08/2010). "Cell Lines" (PDF). Growth and Maintenance of Insect cell lines. Rev. Date - 8 March 2010. Invitrogen. Manual part no. 25-0127, MAN0000030. Retrieved 02/23/2012.