Sodium orthovanadate

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Sodium orthovanadate
Identifiers
CAS number 13721-39-6 YesY
PubChem 61671
ChEMBL CHEMBL179166
RTECS number YW1120000
Jmol-3D images Image 1
Properties
Molecular formula Na3VO4
Molar mass 183.908 g/mol
Appearance white powder
Density 2.16 g/cm³, solid
Melting point 858 °C (1,576 °F; 1,131 K)
Solubility in water 22.17 g/100 mL
Solubility insoluble in ethanol
Structure
Crystal structure cubic
Thermochemistry
Specific
heat capacity
C
164.8 J/mol K
Std molar
entropy
So298
190 J/mol K
Std enthalpy of
formation
ΔfHo298
-1757 kJ/mol
Hazards
Main hazards Harmful.
NFPA 704
Flammability code 1: Must be pre-heated before ignition can occur. Flash point over 93 °C (200 °F). E.g., canola oil Health code 2: Intense or continued but not chronic exposure could cause temporary incapacitation or possible residual injury. E.g., chloroform Reactivity code 0: Normally stable, even under fire exposure conditions, and is not reactive with water. E.g., liquid nitrogen Special hazards (white): no codeNFPA 704 four-colored diamond
Flash point Non-flammable
LD50 330 mg/kg (oral, rat)
Except where noted otherwise, data are given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
 YesY (verify) (what is: YesY/N?)
Infobox references

Sodium orthovanadate is the chemical compound Na3VO4 and contains the tetrahedral VO43−.[1] It is an inhibitor of protein tyrosine phosphatases, alkaline phosphatases and a number of ATPases, most likely acting as a phosphate analogue. The VO43- ion binds reversibly to the active sites of most protein tyrosine phosphatases.

It is often added to buffer solutions that are used in protein analysis in molecular biology. The aim is to preserve the phosphorylation of proteins of interest by inhibiting endogenous phosphatases present in cell lysate mixture. It is commonly used at a final working concentration of 1 - 10 mM. It is toxic when inhaled, swallowed or in contact with skin.

Synthesis[edit]

Sodium orthovanadate is created by dissolving vanadium(V) oxide in a solution of sodium hydroxide.

References[edit]

  1. ^ Greenwood, Norman N.; Earnshaw, Alan (1997). Chemistry of the Elements (2nd ed.). Butterworth-Heinemann. ISBN 0080379419. 
Swarup, G. et al. Biochem. Biophys. Res. Comm. 107: 1104-1109 (1982).

See also[edit]