The St. Louis Limestone is a large geologic formation covering a wide area of the midwest of the United States. It is named after an exposure at St. Louis, Missouri. It consists of sedimentary limestone with scattered chert beds, including the heavily chertified Lost River Chert Bed in the Horse Cave Member. It is exposed at the surface through western Kentucky and Middle Tennessee, including the city of Clarksville, Tennessee. The limestone deposit is Mississippian in age, in the Meramecian series, roughly 300 million years old.
Fossils commonly found in the St. Louis include the rugosan corals Lithostrotion and Lithostrotionella and the bryozoan Fenestrellina.