St Nicholas Shambles was a mediaeval church in the City of London. It was on the corner of Butcher Hall Lane (now King Edward Street) and Newgate Street. It took its name from the Shambles, the butchers area in the west of Newgate Street. The site was extensively excavated in 1975–9 in preparation for the GPO headquarters, now the BT Centre, the headquarters of BT Group.
The excavations in the 1970s identified several phases of building. The original nave and chancel probably dated from the 11th century. They were extended in the late 12th century. Chapels were added to the east end in the 14th century, a north aisle was added to the nave in the first half of the 15th century, and, finally, the east end was rebuilt and a sacristy added on the north. The excavations included the grave yard. Amongst the finds was a woman who died in the later stages of childbirth. 
In 1546, Henry VIII gave the church, along with that of St Ewin (also known as St Audoen) and the dissolved Christ Church priory to the City corporation. A new parish was created for Christ Church, out of those of St Nicholas and St Ewin, and part of that of St Sepulchre. St Nicholas' was demolished in 1547.