As a graduate student at Yerkes Observatory he worked under William Morgan with fellow graduate student Don Osterbrock. In 1952, they published their observations that demonstrated the spiral structure of the Milky Way by estimating the distances to H II regions and young hot stars. For a while Sharpless was at Mount Wilson Observatory where he worked on galaxy photography with Walter Baade and Edwin Hubble.
In 1953 Sharpless joined the staff of the United States Naval Observatory Flagstaff Station. Here he surveyed and cataloged H II regions of the Milky Way Galaxy using the images from the Palomar Sky Survey. From this work Sharpless published his catalog of H II regions in two editions, the first in 1953 with 142 nebula. The second and final edition was published in 1959 with 313 nebulae (see Sharpless catalog).
Stewart Sharpless was before his death a retired Professor Emeritus in the Department of Physics and Astronomy at the University of Rochester.
- Stewart Lane Sharpless
- Sharpless, Stewart Lane
- Shirley K. Cohen, Interview with Donald E. Osterbrock, Feb 10, 2003, California Institute of Technology Archives
- Steven, Dick (2002). Sky and Ocean Joined: The U.S. Naval Observatory, 1830-2000. Cambridge University Press. ISBN 978-0-521-81599-4.
- Stewart Sharpless, A Catalogue of Emission Nebulae Near the Galactic Plane, Astrophysical Journal, vol. 118, p.362, 1953
- Stewart Sharpless, A Catalogue of H II Regions, Astrophysical Journal Supplement, vol. 4, p.257, 1959
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