|Stain of S. mutans in thioglycollate broth culture.|
Streptococcus mutans is facultatively anaerobic, Gram-positive coccus-shaped bacterium commonly found in the human oral cavity and is a significant contributor to tooth decay. The microbe was first described by J Kilian Clarke in 1924.
Streptococcus mutans is a gram-positive organism that is the primary causative agent in the formation of dental cavities in humans. Gram-positive bacteria are those that are stained dark-blue or violet by Gram staining. This is based on the physical properties of their cell walls, as opposed to gram-negative bacteria, which cannot retain the crystal violet stain. Streptococcus is a genus of spherical Gram-positive bacteria belonging to the phylum Firmicutes and the lactic acid bacteria group. S. mutans, a member of the human oral flora, is widely recognized as the main etiological agent of dental cavities.
Conditions in the oral cavity are diverse and complex, frequently changing from one extreme to another. Thus, to survive in the oral cavity, S. mutans must tolerate rapidly harsh environmental fluctuations and exposure to various anti-microbial agents in order to survive. However, the mechanisms under which this cariogenic pathogen can survive and proliferate under such extreme environmental conditions are largely unknown, as little research has been done on this matter.
Twenty-five species of oral streptococci live in the oral cavity. Each species has developed specific specialized properties for colonizing different oral sites and constantly changing conditions to fight competing bacteria and to withstand external challenges. Imbalances in the microbial biota can initiate oral diseases. Under special conditions, commensal streptococci can switch to opportunistic pathogens, initiating disease and damaging the host. Oral streptococci has both harmless and harmful bacteria. "Mutans streptococci" are the most important bacteria associated with tooth decay. S. mutans, the microbial species most strongly associated with carious lesions, is naturally present in the human oral microbiota. The taxonomy of these complex bacteria remains tentative. A 1970’s study found that S. mutans was more prevalent on the pits and fissures, constituting 39% of the total streptococci in the oral cavity. Fewer S. mutans were found on the buccal surface (2–9%).
Role in tooth decay 
Early colonizers of the tooth surface are mainly Neisseria spp. and streptococci, including S. mutans. The growth and metabolism of these pioneer species changes local environmental conditions (e.g., Eh, pH, coaggregation, and substrate availability), thereby enabling more fastidious organisms to further colonize after them, forming dental plaque. Along with S. sobrinus, S. mutans plays a major role in tooth decay, metabolizing sucrose to lactic acid using the enzyme Glucansucrase. The acidic environment created in the mouth by this process is what causes the highly mineralized tooth enamel to be vulnerable to decay. S. mutans is one of a few specialized organisms equipped with receptors that improve adhesion to the surface of teeth. Sucrose is used by S. mutans to produce a sticky, extracellular, dextran-based polysaccharide that allows them to cohere, forming plaque. S. mutans produces dextran via the enzyme dextransucrase (a hexosyltransferase) using sucrose as a substrate in the following reaction:
- n sucrose → (glucose)n + n fructose
However, many other sugars—glucose, fructose, lactose—can be digested by S. mutans, but they produce lactic acid as an end-product. It is the combination of plaque and acid that leads to dental decay. Due to the role the S. mutans plays in tooth decay, there have been many attempts to make a vaccine for the organism. So far, such vaccines have not been successful in humans. Recently, proteins involved in the colonization of teeth by S. mutans have been shown to produce antibodies that inhibit the cariogenic process. A molecule recently synthesized in Yale University and University of Chile called Keep 32 is supposed to be able to kill Streptococcus mutans.
Life in the oral cavity 
Surviving in the oral cavity, S. Mutans is the primary causal agent and the pathogenic species responsible for dental caries (tooth decay or cavities) specifically in the initiation and development stages.
Dental plaque is typically the precursor to tooth decay and contains more than 600 different microorganisms, contributing to the oral cavity’s overall dynamic environment that frequently undergoes rapid changes in pH, nutrient availability, and oxygen tension. Dental plaque adheres to the teeth and consists of bacterial cells while plaque is the biofilm on the surfaces of the teeth. Dental plaque and S. mutans is frequently exposed to "toxic compounds" from oral healthcare products, food additives, and tobacco. Degradation by-products of dental composites resins (fillings) can be another source of toxic chemicals that can interfere with the bacterial growth of S. mutans.
While S. mutans grows in the biofilm, cells maintain a balance of metabolism that involves production and detoxification. Biofilm is an aggregate of microorganisms in which cells adhere to each other or a surface. Bacteria in the biofilm community can actually generate various toxic compounds that interfere with the growth of other competing bacteria. However, there have been very few studies on how S. mutans can tolerate such exposure to various toxic substances during its growth in the oral biofilm and is, thus, poorly understood.
S. mutans has over time developed strategies to successfully colonize and maintain a dominant presence in the oral cavity. The oral biofilm is continuously challenged by changes in the environmental conditions. In response to such changes, the bacterial community evolved with individual members and their specific functions to survive in the oral cavity. S. mutans has been able to evolve from nutrition-limiting conditions to protect itself in extreme conditions. Streptococci represents 20% of the oral bacteria and actually determines the development of the biofilms. Although S. mutans can be antagonized by pioneer colonizers, once they become dominant in oral biofilms, dental caries can develop and thrive.
Survival under stressful conditions 
Transformation is a bacterial adaptation involving the transfer of DNA from one bacterium to another through the surrounding medium (see Transformation (genetics)). Transformation is a primitive form of sex (see Sexual reproduction). For a bacterium to bind, take up, and recombine exogenous DNA into its chromosome it must enter a special physiological state termed “competence” (see Natural competence). In S. mutans, a peptide pheromone quorum-sensing signaling system controls genetic competence. This system functions optimally when the S. mutans cells are in crowded biofilms. S. mutans cells growing in a biofilm are transformed at a rate 10- to 600-fold higher than single cells growing under uncrowded conditions (planktonic cells). Induction of competence appears to be an adaptation for repairing DNA damages caused by crowded, stressful conditions (see Natural competence and Michod et al.).
Cariogenic potential 
The etiological agent of dental caries is associated with its ability to metabolize various sugars, form a robust biofilm, produce an abundant amount of lactic acid, and thrive in the acid environment it generates.
Dental caries is a dental biofilm-related oral disease associated with increased consumption of dietary sugar. When dental biofilms remain on tooth surfaces, along with frequent consumption of sugar, acidogenic bacteria (members of dental biofilms) will metabolize the sugar to organic acids. Persistence of this acidic condition encourages the proliferation of acidogenic and aciduric bacteria as a result of their ability to survive at a low-pH environment. The low-pH environment in the biofilm matrix erodes the surface of the teeth and begins the "initiation" of the dental caries. If the adherence of S. mutans to the surface of teeth or the physiological ability (acidogenity and aciduricity) of S. mutans in dental biofilms can be reduced or eliminated, the acidification potential of dental biofilms and later cavity formations can be decreased.
Susceptibility to disease varies between individuals and immunological mechanisms have been proposed to confer protection or susceptibility to the disease. These mechanisms have yet to be fully elucidated but it seems that while antigen presenting cells are activated by S. mutans in vitro, they fail to respond in vivo. Immunological tolerance to S. mutans at the mucosal surface may make individuals more prone to colonisation with S. mutans and therefore increase susceptibility to dental caries.
In children 
In general, S. mutans is acquired in the oral cavity at the moment of tooth eruption. But S. mutans has been detected in the oral cavity of predentate children. This suggests that the eruption of teeth is not a necessary prerequisite. Thus, this species may not be confined to dental plaque. The adhesion, invasion, and persistence within the oral cells are considered the virulence mechanism of S. mutans to colonize and survive in the oral cavity in the absence of a tooth surface.
Cardiovascular disease 
S. mutans is implicated in the pathogenesis of certain cardiovascular diseases. S. mutans is the most prevalent bacterial species detected in extirpated heart valve tissues as well as in atheromatous plaques, with an incidence of 68.6% and 74.1%, respectively.
Prevention and treatment 
Practice of good oral hygiene including daily brushing, flossing and the use of appropriate mouthwash can significantly reduce the number of oral bacteria and inhibit their proliferation. Oral bacteria often live in plaque, a kind of biofilm and hence mechanical removal of plaque is the most effective way of getting rid of harmful oral bacteria, as bacterial biofilms are notoriously resistant to antibiotics and antimicrobial rinses. However, there are some remedies used in the treatment of oral bacterial infection, in conjunction with mechanical cleaning.
Anti-microbial agents used in dentistry 
Fluoride has a direct inhibitory effect on the enolase enzyme.
Chlorhexidine reduces populations of S. mutans presumably by interfering with bacterial adherence.
Xylitol is a non-cariogenic sugar alcohol found in gum and oral health care products which is not able to be metabolized into the cariogenic acids that commonly cause tooth demineralization and decay. Chewing Xylitol containing gum after each meal stimulates saliva flow (the body's primary tooth protection agent, which washes away food debris from the oral surfaces and provides essential ionic factors that promote remineralization), and helps fight the growth of colonizing, cariogenic bacteria. This is a practice widely encouraged by dental professionals as a protective factor against caries disease.
Quaternary ammonium cation is a nitrogen compound in which a central nitrogen atom is joined to four organic groups.
Green tea extract 
Green tea extract is rich in catechin, a class of antioxidants. Topically applied green tea extract inhibits S. mutans growth, kills oral bacteria, combats oral plaque and inhibits collagenase activity.
Tea tree oil 
Macelignan from nutmeg 
Curcuminoids are the main component of turmeric and have a wide range of pharmacological uses. Many studies of turmeric have revealed antimicrobial properties. Researchers discovered that a fraction could be separated from turmeric and showed that it had anti-biofilm activity. Researchers based this on a comparison of curcuminoid content and anti-acidogenic activity against S. mutans. The data showed that the separated turmeric fraction and curcuminoids may be effective in controlling dental biofilms and dental cavity formations, as dental biofilms and dental cavity formations are related.
Barley tea 
In January of 2011 scientists developed safe and effective sugar-free herbal lollipops that kill cavity-causing bacteria. As already established, tooth decay is caused by cariogenic bacteria like S. mutans. S. mutans converts sugars into acids that dissolve minerals in the tooth enamel. A previous study found that novel compound from the extraction of licorice roots, Glycyrrhizol A, has strong antimicrobial activity against cariogenic bacteria. Killing such bacteria would control or prevent tooth decay. Researchers produced specific herbal extracts to develop a sugarfree lollipop to kill bacteria such as S. mutans. Subsequent studies on humans showed a reduction of cariogenic bacteria in the oral cavity after eating these lollipops.[dead link]
Keep 32 
Keep 32, a topical antibiotic developed in 2012, has been claimed to kill S. mutans in 60 seconds.
Smart Bomb 
Smart Bomb Mouthwash, an antimicrobial product that claims to target and kills S. mutans and not beneficial bacteria in the mouth for 4 days.
See also 
- Dental caries
- Mutacin 1140
- Oral microbiology
- Streptococcus viridans
- Xylitol reduces Streptococcus
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