Sudan Black B

Sudan Black B
IUPAC name (2,2-dimethyl-1,3-dihydroperimidin-6-yl)-(4-phenylazo-1-naphthyl)diazene
Other names Sudan Black; Fat Black HB; Solvent Black 3; C.I. 26150
Identifiers
CAS number	4197-25-5 Y
PubChem	61336
ChemSpider	55272 Y
MeSH	Sudan+Black+B
Jmol-3D images	Image 1
SMILES N(=N/c5c1c(cccc1)c(/N=N/c4c2cccc3NC(Nc(c23)cc4)(C)C)cc5)\c6ccccc6
InChI InChI=1S/C29H24N6/c1-29(2)30-26-14-8-13-22-25(17-18-27(31-29)28(22)26)35-34-24-16-15-23(20-11-6-7-12-21(20)24)33-32-19-9-4-3-5-10-19/h3-18,30-31H,1-2H3/b33-32+,35-34+ Y Key: YCUVUDODLRLVIC-VPHDGDOJSA-N Y InChI=1/C29H24N6/c1-29(2)30-26-14-8-13-22-25(17-18-27(31-29)28(22)26)35-34-24-16-15-23(20-11-6-7-12-21(20)24)33-32-19-9-4-3-5-10-19/h3-18,30-31H,1-2H3/b33-32+,35-34+ Key: YCUVUDODLRLVIC-VPHDGDOJBF
Properties
Molecular formula	C29H24N6
Molar mass	456.54 g/mol
Melting point	120–124 °C
Y (verify) (what is: Y/N?) Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa)
Infobox references

Sudan Black B (C₂₆H₂₄N₄O) is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. It has the appearance of a dark brown to black powder with maximum absorption at 596-605 nm and melting point 120–124 °C. It stains blue-black.

Sudan Black B is one of the dyes used for Sudan staining. Similar dyes include Oil Red O, Sudan III, and Sudan IV.

Sudan Black B can be used to stain some other materials than the other Sudan dyes, as it is not so specific to lipids.

A use of Sudan Black B is in fingerprint enhancement. It is useful for detecting fats that are contaminated with oil and grease.

In differentiating haematological disorders Sudan black will stain myeloblasts but not lymphoblasts.

External links

• Stains File entry