TRAMP complex

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The TRAMP complex (Trf4/Air2/Mtr4p Polyadenylation complex) is a multi-protein complex consisting of the RNA helicase (Mtr4/Dob1), a poly(A) polymerase (PAP) (either Trf4 or Trf5) and a zinc knuckle protein (either Air1 or Air2). The TRAMP complex interacts with the exosome complex in the nucleus of eukaryotic cells and is involved in the 3' end processing and degradation of ribosomal RNA and snoRNAs.[1][2][3]

Originally discovered in the yeast Saccharomyces cerevisiae, the TRAMP complex trims the poly(A) tails of RNAs destined for Rrp6 and the core exosome down to 4-5 adenosines,[1][4] assisting in transcript recognition and exosome complex activation. In this way, TRAMP plays a critical role in ridding the cell of noncoding transcripts generated through pervasive RNA polymerase II transcription, as well as functioning in the biogenesis and turnover of functional coding and noncoding RNAs.[5][6]

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  1. ^ a b Jia, Huijue; Wang, Xuying; Liu, Fei; Guenther, Ulf-Peter; Srinivasan, Sukanya; Anderson, James T.; Jankowsky, Eckhard (June 2011). "The RNA Helicase Mtr4p Modulates Polyadenylation in the TRAMP Complex". Cell 145 (6): 890–901. doi:10.1016/j.cell.2011.05.010. PMID 21663793. Retrieved 16 December 2014. 
  2. ^ LaCava, John; Houseley, Jonathan; Saveanu, Cosmin; Petfalski, Elisabeth; Thompson, Elizabeth; Jacquier, Alain; Tollervey, David (3 June 2005). "RNA Degradation by the Exosome Is Promoted by a Nuclear Polyadenylation Complex". Cell 121 (5): 713–724. doi:10.1016/j.cell.2005.04.029. PMID 15935758. Retrieved 16 December 2014. 
  3. ^ Vaňáčová, Štěpánka; Wolf, Jeannette; Martin, Georges; Blank, Diana; Dettwiler, Sabine; Friedlein, Arno; Langen, Hanno; Keith, Gérard; Keller, Walter (19 April 2005). "A New Yeast Poly(A) Polymerase Complex Involved in RNA Quality Control". PLoS Biology 3 (6): e189. doi:10.1371/journal.pbio.0030189. Retrieved 16 December 2014. 
  4. ^ Callahan, Kevin P; Butler, J Scott (2 December 2009). "TRAMP Complex Enhances RNA Degradation by the Nuclear Exosome Component Rrp6". Journal of Biological Chemistry 285 (6): 3540–3547. doi:10.1074/jbc.M109.058396. PMC 2823493. PMID 19955569. Retrieved 17 December 2014. 
  5. ^ Ciais, Delphine; Bohnsack, Markus T; Tollervey, David (16 March 2008). "The mRNA encoding the yeast ARE-binding protein Cth2 is generated by a novel 3' processing pathway". Nucleic Acids Research 36 (9): 3075–3084. doi:10.1093/nar/gkn160. Retrieved 16 December 2014. 
  6. ^ Grzechnik, Pawel; Kufel, Joanna (October 2008). "Polyadenylation Linked to Transcription Termination Directs the Processing of snoRNA Precursors in Yeast". Molecular Cell 32 (2): 247–258. doi:10.1016/j.molcel.2008.10.003. PMID 18951092. Retrieved 16 December 2014.