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TWL may refer to:
- Thermal work limit, a heat index to prevent heat stress
- Official Tournament and Club Word List, the official word authority for tournament Scrabble in North America
- TeamWarfare League, a community based gaming website
- Transepidermal water loss, water loss across epidermis
- The Tony Williams Lifetime, a jazz-rock fusion group led by jazz drummer Tony Williams
- Themable Widget Library, a Java library for creating user interfaces with OpenGL
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