Talk:Acid-fast

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Headline text[edit]

wt questions r asked during the performance of acid fast staining -- —The preceding unsigned comment was added by 221.134.253.77 (talkcontribs).

  • I'm sorry, what about acid fast staining did you want more info on? -- MarcoTolo 06:33, 18 February 2007 (UTC)


Hi. Shouldn't Rhodococcus, Tsukamurella and Gordonia also be on the list of (partially-) acid-fast bacteria? --217.68.116.150 (talk) 13:07, 3 October 2011 (UTC)

Merger proposal[edit]

I believe that information at Ziehl–Neelsen stain probably belongs in this article. As of right now, this article actually has more useful information on the staining procedure and the Ziehl–Neelsen stain is not notable for anything other than checking for acid-fastness. Plus, some information from the Ziehl–Neelsen stain article can be removed. Thoughts? The Haz talk 03:22, 16 November 2011 (UTC)

I Differ:
Acid fast staining is not a negligibly small subject and there are so many stains and so many variations and modifications. Acid fastness is a property of many microorganisms and the general principles of it should be discused here.. What is acid fast. What is modified acid fast (nocardia etc) what are the stains what are the modifications what are their basic comparisons do need to be here.. and then how to identify different types of MOTT (mycobacteria other than TB) by the staining characteristics. I thinkZiehl–Neelsen stain, Kinyoun stain, Fite stain, Ellis and Zabrowarny stain (excludes phenol), Auramine-rhodamine stain, Auramine phenol stain all probably deserve a entry on their own. If not all of them, at least Z-N definitely does as there are literally thousands of scientific publications on it. Each of the entries should have a cross reference to each other. If Fite cannot grow beyond a stub it may be a subsection under ZN. But Kinyoun is already an entry and I think it deserved to be. It is fairly different from ZN and it is important for people to learn about this choice. (it reduces fire hazard)--Dr.saptarshi (talk) 17:55, 28 June 2012 (UTC)
The dako guide says "the Fite stain is used for staining of M.leprae which has cell walls that are more susceptible to damage in the deparaffinization process. the Fite procedure thus includes peanut oil in the deparaffinization solvent to protect the bacterial cell wall. the acid used for decolorization in the Fite procedure is also weaker". Actually daco guide is talking about one subtype of Fite faraco. Per the stains file there are several variations of Fite, like Fite-Faraco Staining and Wade Fite staining which mainly differ in the dewaxing . --Dr.saptarshi (talk) 18:31, 28 June 2012 (UTC)
IHC world gives one of the original references:
  1. Faraco 1938 (?)
  2. Fite, G.L., Cambre, F.J. and Turner, M.H. 1947, Procedures for demonstrating lepra bacilli in paraffin sections. Archives of Pathology, V43, p624-625

--Dr.saptarshi (talk) 18:44, 28 June 2012 (UTC)

The terms should not be murged, as AFS (acid fast stain) is used generally for various species (e.g. weak in Nocardia & Rhodococcus or even some protozoa), contrary to Ziehl - Neelsen that is AFS used for Mycobacterium spp'[edit]

The term Ziehl–Neelsen stain refers to the identification of Mycobacterium spp such as M. tuberculosis and M. leprae. AFS (acid fast stain) as a term generally refers to the identification of many microbial species beyond Mycobacterium species. For example it is used for Cryptosporidium parvum (a protozoan) or Nocardia staining! In Nocardia and Rhodococcus the stain is modified (to 1% H2SO4). 688dim (talk) 13:51, 6 January 2015 (UTC)