Talk:Alternative splicing

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nice work, people[edit]

Wonderful article. Thanks to all who have contributed. JMBurke1791 (talk) 22:44, 14 December 2010 (UTC)

thyroid hormone calcitonin?[edit]

In the discovery section it is mentioned "thyroid hormone calcitonin" as one of the first examples of alternative splicing found. but calcitonin is not a thyroid hormone. it is a hormone secreted by the parathyroid gland, which is a different thing. —Preceding unsigned comment added by 121.2.225.207 (talk) 15:01, 10 October 2009 (UTC)

transcripts diversity[edit]

I would start by stating something like this:

Alternative splicing is one of the main mechanisms that eukaryotic cells have evolved to generate a great diversity of functional transcripts from a fixed number of genes.


Its role has been particuarly studied and understood after the publication of the human genome and the subsequent debates about the number of genes in the human specie. It has become clear that many of the most complex organisms tend to have a smaller number of genes than expected, and that mechanisms like alternative splicing help to generate diversity.

What do you think? Dalloliogm 14:23, 10 October 2007 (UTC)

For the first sentence, more like

Alternative splicing is a mechanism that eukaryotic cells have evolved to generate a great diversity of functional messenger RNAs, encoding multiple functional proteins, from a fixed number of genes.

I think it's especially important to say that it generates diverse mRNAs, rather than transcripts. The transcript is the immediate product of transcription, and that's the same all the time -- after splicing you have a messenger RNA, and those are what are diverse because of alternative splicing. Agathman (talk) 00:41, 24 April 2009 (UTC)

I think you are confusing transcript with primary transcript. Narayanese (talk) 18:23, 24 April 2009 (UTC)
Okay, but I don't find that the use of the terms is really consistent in the literature. Perhaps the best usage would be "primary transcript" and "mRNA", thus avoiding the potential for confusion. Agathman (talk) 18:29, 24 April 2009 (UTC)

SR proteins[edit]

"The regulation and selection of splice sites is done by Serine/Arginine-residue proteins, or SR proteins."

Are you sure of this? I think there are other class of proteins involved, like hnRNPs.

Dalloliogm 14:23, 10 October 2007 (UTC)

Alternative promotor mechanism[edit]

This is not splicing mechanism. neffk 04:24, 29 August 2006 (UTC)

Alternative poly-A mechanism[edit]

I have not heard of this... RNA polymerase II and cleavage, polyadenylation specificity factor (CPSF), and cleavage stimulatory factor (CStF) terminate transcription 10-30 nt downstream of the AAUAAA transcription stop sequence. Poly-A tail is produced by PAP (poly-A polymerase). Unless scholarly research can be referenced, I think this should be removed. neffk 04:22, 29 August 2006 (UTC)

nonfunctional alternative splicing[edit]

The article contains no indication that alternatively spliced variants might be anything other than adaptive. It's an issue that's currently in need of emphasis in the field. Alternative splicing can clearly be regulated and adaptive, but essentially all of the diagrammed forms of alternative configurations for a transcript can also occur as a result of abberant transcript processing resulting from exon skipping, incorrect choice of cryptic splice sites, etc. For example, perhaps 1/3 to 1/2 (possibly more) of alternatively spliced variants in humans contain premature termination codons and are almost certainly nonfunctional. Unfortunately, a general belief perhaps uncharitably characterized by "if the cell produces it, it must be good" has led to coupling this mechanism to nonsense-mediated decay in proposals of a new form of gene regulation, when really the most parsimonious explanation is that NMD is just doing its job in removing these incorrectly processed transcripts. Dgscofield 02:51, 28 October 2006 (UTC)

clean-up[edit]

i tagged this article for clean-up since it is written in rather colloquial language, and since it lacks interesting details such as the explanation of trans- and cis regulatory elements, which influence the regulation of alternative splicing. Further, in this article many facts refer to splicing in general, which can be left out or should be included in the article "splicing (genetics)". —The preceding unsigned comment was added by Inside x (talkcontribs) 15:06, 27 April 2007 (UTC).

Intron Retention mode[edit]

However, the intron must be properly encoding for amino acids. The intron's code must be properly expressible, otherwise a stop codon or a shift in the reading frame will cause the protein to be non-functional.

This is not necessarly true: in fact, a retained intron can also occur in one of the Non Translated Regions (NTRs) on the upstream or the downstream of the transcript.

My beef with this is that if "the intron must be properly encoding for amino acids", does that not, by definition, make it an exon? Or is this trying to say that retained introns, such as those in the UTR's, must not interfere with the transcription machinery(stop codon etc)? Because it seems to imply that "coding introns" have to ...code properly. Geno-Supremo (talk) 16:32, 9 November 2008 (UTC)

First discovered in.. ?[edit]

I wonder who and when first discovered alt. splicing. --CopperKettle (talk) 00:35, 1 January 2009 (UTC)