Talk:Minisatellite
| WikiProject Genetics | |||||||||||||||||
|
|||||||||||||||||
| WikiProject Molecular and Cellular Biology | (Rated B-class, Mid-importance) | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
|||||||||||||||||
Isn't this synonymous with "VNTR" (variable number tandem repeat) ?.. Or are they different?
--Hughitt1 04:25, 18 December 2005 (UTC)
Contents |
[edit] microsatellites and minisatellites
Aren't they the same thing? I'd really appreciate if someone defines on each page the difference between those two and VNTR if there are any
[edit] Microsatellites and minisatellites
24.18.234.112 (talk)These sequences are both tandem repeated segments of DNA. The only difference is the length of the repeated subunit. When the subunit is between 1 and 9 basepairs, it is called a microsatellite. Minisatellites are composed of longer subunits of at least 10 base pairs in length. For example ATATATATATATATATAT can be written (AT)n and is a microsatellite. A minisatellite might be something like (AGGCGAGGGTTG)n. The minimum number of repeats required to be considered a "satellite" varies, depending on the type (eg. (AT)n versus (ATTTTTTTTTT)n). Generally, the subunit must be repeated at least 6 times to be considered a tandem repeat. See http://tandem.bu.edu/trf/trf.html. I hope this helps, I do not usually edit wiki entries. 24.18.234.112 (talk) 00:17, 22 June 2010 (UTC)
Microsatellites are shorter repeats, most typically 1-2 basepairs long. Microsatellites are caused by repeat slippage which is limited to offsets of only a couple of bases.
"1 basepair long" microsatellite does not make very much sense, it's a single nucleotide run. Most microsatellites are 2-4 bp long —Preceding unsigned comment added by 151.141.84.173 (talk) 23:20, 14 January 2009 (UTC)
[edit] micro and mini
Ellegren, 2004 has a nice little explanation:
What is a microsatellite? Genomes are scattered with simple repeats. Tandem repeats occur in the form of iterations of repeat units of almost anything from a single base pair to thousands of base pairs.Mono-, di-, triand tetranucleotide repeats are the main types of microsatellite, but repeats of five (penta-) or six (hexa-) nucleotides are usually classified as microsatellites as well. Repeats of longer units form minisatellites or, in the extreme case, satellite DNA. The term satellite DNA originates from the observation in the 1960s of a fraction of sheared DNA that showed a distinct buoyant density, detectable as a ‘satellite peak’ in DENSITY GRADIENT CENTRIFUGATION, and that was subsequently identified as large centromeric tandem repeats.When shorter (10–30-bp) tandem repeats were later identified, they came to be known as minisatellites. Finally,with the discovery of tandem iterations of simple sequence motifs, the term microsatellites was coined. The difference between the terms micro- and minisatellites might not be obvious per se, but it is motivated by the difference in the mutational mechanisms of repeats of just a few nucleotides and of ten or more —Preceding unsigned comment added by 59.167.67.245 (talk) 05:05, 18 April 2008 (UTC)
==Funny Memory hook==
"Pure as gold" and "cut the pie" may be funny memory hooks, but why are the in the article "microsatellites", not in "Nucleic acid"? 134.2.164.11 (talk) 19:42, 9 December 2008 (UTC)
[edit] First time used
What on earth is that paragraph doing? its incredibly poorly written, Im not going to attempt to re-write it as I dont know enough about the case but I think it needs either removed or completely re-written —Preceding unsigned comment added by 192.173.4.218 (talk) 13:38, 3 May 2009 (UTC)
