SH 326 begins in East Texas at a junction with US 90 and FM 365 in Nome. It heads north from this junction to an intersection with SH 105 in Sour Lake. The highway continues to the north to an intersection with FM 421. Heading north, the highway continues to a junction with FM 770. The highway intersects FM 1293 as it enters the Kountze city limits. SH 326 reaches its northern terminus at US 69 and US 287 in Kountze.