The Mothership Connection — Live from Houston is divided evenly between studio and live performances. Side one consists of a live recording made at a concert at The Summit in Houston, Texas, on October 31, 1976. Side two is made up of previously released material from Clinton's solo albums, including the hit single "Atomic Dog".
In conjunction with the release of The Mothership Connection — Live from Houston, a videotape of the live performance was released. The video also included music videos for several studio tracks. The live performance was released in its entirety in 1998 by Pioneer Artists.