Thermus thermophilus

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Thermus thermophilus
Scientific classification
Kingdom: Bacteria
Phylum: Deinococcus-Thermus
Class: Deinococci
Order: Thermales
Family: Thermaceae
Genus: Thermus
Species: T. thermophilus
Binomial name
Thermus thermophilus

Thermus thermophilus is a Gram negative eubacterium used in a range of biotechnological applications, including as a model organism for genetic manipulation, structural genomics, and systems biology. The bacterium is extremely thermophilic, with an optimal growth temperature of about 65 °C (149 °F). Thermus thermophilus was originally isolated from a thermal vent within a hot spring in Izu, Japan by Tairo Oshima and Kazutomo Imahori.[1] The organism has also been found to be important in the degradation of organic materials in the thermogenic phase of composting.[2] T. thermophilus is classified into several strains, of which HB8 and HB27 are the most commonly used in laboratory environments. Genome analyses of these strains were independently completed in 2004.[3]

Biotechnological applications of Thermus thermophilus enzymes[edit]

  • rTth DNA polymerase is a recombinant thermostable DNA polymerase derived from Thermus thermophilus, with optimal activity at 70-80ºC, used in some PCR applications. The enzyme possesses efficient reverse transcriptase activity in the presence of manganese.

External links[edit]

References[edit]

  1. ^ Oshima & Imahori; Imahori, K. (1974). "Description of Thermus thermophilus (Yoshida and Oshima) comb. nov., a nonsporulating thermophilic bacterium from Japanese thermal spa.". Int J Syst Bacteriol. 24: 102–112. doi:10.1099/00207713-24-1-102. 
  2. ^ Beffa, T; Blanc, M; Lyon, PF; Vogt, G; Marchiani, M; Fischer, JL; Aragno, M (1996). "Isolation of Thermus strains from hot composts (60ºC-80ºC)". Appl. Environ. Microbiol. 62 (5): 1723–1727. PMC 167946. PMID 8633870. 
  3. ^ Henne A et al. (May 2004). "The genome sequence of the extreme thermophile Thermus thermophilus". Nat Biotechnol. 22 (5): 547–53. doi:10.1038/nbt956. PMID 15064768.