Holme was born in Lancashire, England on November 3, 1624, to a yeoman named George and his wife Alice. He married Sarah Croft in 1649, and soon enlisted in the army under the leadership of Oliver Cromwell, where he attained the rank of captain. It may have been in the army that he gained some experience in surveying. After retiring he was granted more than 4,000 acres (16 km²) in County Wexford, Ireland, which was under the control and colonization of the English at the time.
At some point he joined the Quaker movement in Ireland. There he met fellow Quaker William Penn, founder of Pennsylvania. In 1682, Penn wrote to Holme from the colony asking him to come be his surveyor, since his original surveyor, Captain William Crispin, had recently become ill and died on the voyage to America. Shortly after, Holme sailed to America.
Holme designed the plan of the city of Philadelphia and produced the first detailed map of Pennsylvania, entitled "A Mapp of Ye Improved Part of Pensilvania in America, Divided Into Countyes, Townships and Lotts...." (published circa 1687). On Penn's arrival in the colony, he appointed Holme as one of his councilors.
Holme held the office of Surveyor-General until his death at the age of 71 in the spring of 1695, in Dublin Township, Philadelphia County (now the Holmesburg section of the city of Philadelphia). In 1863, a memorial was erected at his burial site, in the form of a six-foot-tall marble obelisk, near where his home is believed to have been located, now part of Pennypack Park.
Holme Avenue, Holme Circle, and Thomas Holme Public School were all named in his honor.
- Corcoran, Irma (1992). Thomas Holme, 1624-1695: Surveyor General of Pennsylvania, Volume 200 of Memoirs of the American Philosophical Society, American Philosophical Society. Diane Publishing. p. 317. ISBN 978-0-87169-200-9.
- Hough, Oliver, "Captain Thomas Holme, Surveyor-General of Pennsylvania and Provincial Councillor," The Pennsylvania Magazine of History and Biography, Vol. XIX & XX, 1895 & 1896.
- Biographical essay in Professional Surveyor magazine:
- Image of one of Holme's maps from the Lower Merion Historical Society
- "Portraiture of the City of Philadelphia" (1683), by Thomas Holme
- Brief biography at virtualology.com
- Thomas Holme at Find a Grave