The Toolamba–Echuca railway is a cross-country link between the named towns. As a railway route to and from Echuca, it provides an alternative to the usual route via Bendigo. The line has not been used for passenger services since 1981, and goods movements on the line are intermittent, with it being booked out of service at times. The line was re-opened for goods traffic while there was track work on the line between Seymour and Shepparton.
On 3 October 2013 the line was re-opened for goods traffic.
The last passenger service from Echuca station along the Toolamba–Echuca line ran on 2 March 1981 with Y class diesel locomotive Y161, an ABE carriage and a C van. This consist had only been introduced a few months prior, with a DERM usually being rostered. Toolamba finally closed as a station on 20 December 1987.
During the Regional Fast Rail project, the line was used while work was being undertaken on the main line to Echuca via Bendigo. The line was also used by Freight Australia to send rice traffic from north of Echuca into Melbourne. In 2013 the line was re-instated for use by goods trains after a upgrade to the line.
There have been plans for the line to be converted to standard gauge to provide standard gauge access to Echuca, but they have not yet eventuated.
The Toolamba-Echuca line joins the main line at Toolamba
Lines towards Echuca and Shepparton diverge at Toolamba