Genetically modified crops

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Genetically modified crops (GMCs, GM crops, or biotech crops) are plants used in agriculture, the DNA of which has been modified using genetic engineering techniques. In most cases the aim is to introduce a new trait to the plant which does not occur naturally in the species. Examples in food crops include resistance to certain pests, diseases, or environmental conditions, reduction of spoilage, or resistance to chemical treatments (e.g. resistance to a herbicide), or improving the nutrient profile of the crop. Examples in non-food crops include production of pharmaceutical agents, biofuels, and other industrially useful goods, as well as for bioremediation.

Farmers have widely adopted GM technology. Between 1996 and 2011, the total surface area of land cultivated with GM crops had increased by a factor of 94, from 17,000 square kilometers (4,200,000 acres) to 1,600,000 km2 (395 million acres). 10% of the world's crop lands were planted with GM crops in 2010. As of 2011, 11 different transgenic crops were grown commercially on 395 million acres (160 million hectares) in 29 countries.

There is broad scientific consensus that food on the market derived from GM crops poses no greater risk to human health than conventional food.[1][2][3][4][5] GM crops also provide a number of ecological benefits.[6] However, opponents have objected to GM crops per se on several grounds, including environmental concerns, whether food produced from GM crops is safe, whether GM crops are needed to address the world's food needs, and economic concerns raised by the fact these organisms are subject to intellectual property law.

Gene transfer in nature and traditional agriculture[edit]

Scientists first discovered that DNA naturally transfers between organisms in 1946.[7] It is now known that there are several natural mechanisms for flow of genes, or (horizontal gene transfer), and that these occur in nature on a large scale – for example, it is a major mechanism for antibiotic resistance in pathogenic bacteria, and it occurs between plant species.[8] This is facilitated by transposons, retrotransposons, proviruses and other mobile genetic elements that naturally translocate to new sites in a genome.[9][10] They often move to new species over an evolutionary time scale[11] and play a major role in dynamic changes to chromosomes during evolution.[12][13]

The introduction of foreign germplasm into crops has been achieved by traditional crop breeders by artificially overcoming fertility barriers. A hybrid cereal was created in 1875, by crossing wheat and rye.[14] Since then important traits have been introduced into wheat, including dwarfing genes and rust resistance.[15] Plant tissue culture and the induction of mutations have also enabled humans to artificially alter the makeup of plant genomes.[16][17]

History[edit]

The first genetically modified plant was produced in 1982, using an antibiotic-resistant tobacco plant.[18] The first field trials of genetically engineered plants occurred in France and the USA in 1986, when tobacco plants were engineered to be resistant to herbicides.[19] In 1987, Plant Genetic Systems (Ghent, Belgium), founded by Marc Van Montagu and Jeff Schell, was the first company to develop genetically engineered (tobacco) plants with insect tolerance by expressing genes encoding for insecticidal proteins from Bacillus thuringiensis (Bt).[20] The People’s Republic of China was the first country to allow commercialized transgenic plants, introducing a virus-resistant tobacco in 1992,[21] which was withdrawn from the market in China in 1997.[22]:3 The first genetically modified crop approved for sale in the U.S., in 1994, was the FlavrSavr tomato, which had a longer shelf life, as it took longer to soften after ripening.[23] In 1994, the European Union approved tobacco engineered to be resistant to the herbicide bromoxynil, making it the first commercially genetically engineered crop marketed in Europe.[24] In 1995, Bt Potato was approved safe by the Environmental Protection Agency, making it the first pesticide producing crop to be approved in the USA.[25] The following transgenic crops also received marketing approval in the US in 1995: canola with modified oil composition (Calgene), Bacillus thuringiensis (Bt) corn/maize (Ciba-Geigy), cotton resistant to the herbicide bromoxynil (Calgene), Bt cotton (Monsanto), soybeans resistant to the herbicide glyphosate (Monsanto), virus-resistant squash (Asgrow), and additional delayed ripening tomatoes (DNAP, Zeneca/Peto, and Monsanto).[19] As of mid-1996, a total of 35 approvals had been granted to commercially grow 8 transgenic crops and one flower crop of carnations, with 8 different traits in 6 countries plus the EU.[19] In 2000, with the production of golden rice, scientists genetically modified food to increase its nutrient value for the first time.

Methods[edit]

Plants (Solanum chacoense) being transformed using agrobacterium

Genetically engineered plants are generated in a laboratory by altering their genetic makeup. This is usually done by adding one or more genes to a plant's genome using genetic engineering techniques.[26] Most genetically modified plants are generated by the biolistic method (particle gun) or by Agrobacterium tumefaciens mediated transformation. Plant scientists, backed by results of modern comprehensive profiling of crop composition, point out that crops modified using GM techniques are less likely to have unintended changes than are conventionally bred crops.[27][28]

In research tobacco and Arabidopsis thaliana are the most genetically modified plants, due to well developed transformation methods, easy propagation and well studied genomes.[29][30] They serve as model organisms for other plant species.

In the biolistic method, DNA is bound to tiny particles of gold or tungsten which are subsequently shot into plant tissue or single plant cells under high pressure. The accelerated particles penetrate both the cell wall and membranes. The DNA separates from the metal and is integrated into plant genome inside the nucleus. This method has been applied successfully for many cultivated crops, especially monocots like wheat or maize, for which transformation using Agrobacterium tumefaciens has been less successful.[31] The major disadvantage of this procedure is that serious damage can be done to the cellular tissue.

Agrobacteria are natural plant parasites, and their natural ability to transfer genes provides another method for the development of genetically engineered plants. To create a suitable environment for themselves, these Agrobacteria insert their genes into plant hosts, resulting in a proliferation of plant cells near the soil level (crown gall). The genetic information for tumour growth is encoded on a mobile, circular DNA fragment (plasmid). When Agrobacterium infects a plant, it transfers this T-DNA to a random site in the plant genome. When used in genetic engineering the bacterial T-DNA is removed from the bacterial plasmid and replaced with the desired foreign gene. The bacterium is a vector, enabling transportation of foreign genes into plants. This method works especially well for dicotyledonous plants like potatoes, tomatoes, and tobacco. Agrobacteria infection is less successful in crops like wheat and maize.

Introducing new genes into plants requires a promoter specific to the area where the gene is to be expressed. For instance, if we want the gene to be expressed only in rice grains and not in leaves, then an endosperm-specific promoter would be used. The codons of the gene must also be optimized for the organism due to codon usage bias. The transgenic gene products should also be able to be denatured by heat so that they are destroyed during cooking.

Glyphosate resistance[edit]

One of the most famous kinds of GM crops are "Roundup Ready", or glyphosate-resistant trait. Glyphosate, (the active ingredient in Roundup) kills plants by interfering with the shikimate pathway in plants, which is essential for the synthesis of the aromatic amino acids phenylalanine, tyrosine and tryptophan. More specifically, glyphosate inhibits the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS).

The rationale behind developing this trait, was that the selective herbicides used on grain and grass crops at the time were highly toxic, and not effective against narrow leaved weeds. Thus, developing crops that could withstand spraying with glyphosate would both reduce environmental and health risks, and give an agricultural edge to the farmer.[32]

The shikimate pathway is not present in animals, which instead obtain aromatic amino acids from their diet.

Some micro-organisms have a version of EPSPS that is resistant to glyphosate inhibition. One of these was isolated from an Agrobacterium strain CP4 (CP4 EPSPS) that was resistant to glyphosate.[33][34] This CP4 EPSPS gene was cloned and transfected into soybeans. The CP4 EPSPS gene was engineered for plant expression by fusing the 5' end of the gene to a chloroplast transit peptide derived from the petunia EPSPS. This transit peptide was used because it had shown previously an ability to deliver bacterial EPSPS to the chloroplasts of other plants. The plasmid used to move the gene into soybeans was PV-GMGTO4. It contained three bacterial genes, two CP4 EPSPS genes, and a gene encoding beta-glucuronidase (GUS) from Escherichia coli as a marker. The DNA was injected into the soybeans using the particle acceleration method. Soybean cultivar A54O3 was used for the transformation. The expression of the GUS gene was used as the initial evidence of transformation. GUS expression was detected by a staining method in which the GUS enzyme converts a substrate into a blue precipitate. Those plants that showed GUS expression were then taken and sprayed with glyphosate, and their tolerance was tested over many generations.

Types of genetic engineering[edit]

Transgenic maize containing a gene from the bacteria Bacillus thuringiensis

Transgenic[edit]

Transgenic plants have genes inserted into them that are derived from another species. The inserted genes can come from species within the same kingdom (plant to plant) or between kingdoms (for example, bacteria to plant). In many cases the inserted DNA has to be modified slightly in order to correctly and efficiently express in the host organism. Transgenic plants are used to express proteins like the cry toxins from Bacillus thuringiensis, herbicide resistant genes and antigens for vaccinations[35] Transgenic carrots have been used to produce the drug Taliglucerase alfa which is used to treat Gaucher's disease.[36] In the laboratory, transgenic plants have been modified to increase their photosynthesis (currently about 2% at most plants to the theoretic potential of 9–10%.[37] This is possible by changing the rubisco enzyme (i.e. changing C3 plants into C4 plants[38]), by placing the rubisco in a carboxysome, by adding CO
2
pumps in the cell wall,[39][40] by changing the leaf form/size.[41][42][43][44] Plants have been engineered to exhibit bioluminescence which might one day be a sustainable alternative to electric lighting.[45] Still other transgenic plants have been modified to fix ambient nitrogen in the plant.[46]

Cisgenic[edit]

Cisgenic plants are made using genes found within the same species or a closely related one, where conventional plant breeding can occur. Some breeders and scientists argue that cisgenic modification is useful for plants that are difficult to crossbreed by conventional means (such as potatoes), and that plants in the cisgenic category should not require the same level of legal regulation as other genetically modified organisms.[47]

Subgenic[edit]

In 2014, Chinese researcher Gao Caixia filed patents on the creation of a strain of wheat that is resistant to powdery mildew. The strain lacks genes that encode proteins that repress defenses against the mildew. The researchers deleted all three copies of the genes from wheat's hexaploid genome. The strain promises to reduce or eliminate the heavy use of fungicides to control the disease. Gao used the TALENs and CRISPR gene editing tools without adding or changing any other genes. No field trials are yet planned.[48][49]

Business of GM Crops[edit]

The global value of biotech seed alone was US$13.2 billion in 2011, with the end product of commercial grain from biotech maize, soybean grain and cotton valued at approximately US$160 billion or more per year.[50]

Players in agriculture business markets include seed companies, agrochemical companies, distributors, farmers, grain elevators, and universities that develop new crops and whose agricultural extensions advise farmers on best practices.

The largest share of the GMO crops planted globally are from seed created by the United States firm Monsanto.[51] In 2007, Monsanto's trait technologies were planted on 246 million acres (1,000,000 km2) throughout the world, a growth of 13 percent from 2006. However, patents on the first Monsanto products to enter the marketplace will begin to expire in 2014, democratizing Monsanto products. Syngenta, DuPont (especially via its Pioneer Hi-Bred subsidiary), and Bayer CropScience are also major players in the US and Europe. In addition, a 2007 report from the European Joint Research Commission predicts that by 2015, more than 40 per cent of new GM plants entering the global marketplace will have been developed in Asia.[52]

In the corn market, Monsanto's triple-stack corn—which combines Roundup Ready 2-weed control technology with YieldGard (Bt) Corn Borer and YieldGard Rootworm insect control—is the market leader in the United States. U.S. corn farmers planted more than 32 million acres (130,000 km2) of triple-stack corn in 2008,[53] and it is estimated the product could be planted on 56 million acres (230,000 km2) in 2014–2015. In the cotton market, Bollgard II with Roundup Ready Flex was planted on approximately 5 million acres (20,000 km2) of U.S. cotton in 2008.[54]

According to the International Service for the Acquisition of Agri-Biotech Applications (ISAAA), in 2010 approximately 15 million farmers grew biotech crops in 29 countries. Over 90% of the farmers were resource-poor in developing countries.[55] 6.5 million farmers in China and 6.3 million small farmers in India grew biotech crops (mostly Bacillus thuringiensis cotton). The Philippines, South Africa (biotech cotton, maize, and soybeans often grown by subsistence women farmers) and another twelve developing countries also grew biotech crops in 2009.[56] 10 million more small and resource-poor farmers may have been secondary beneficiaries of Bt cotton in China.

According to a review published in 2012 and based on data from the late 1990s and early 2000s, much of the GM crop grown each year is used for livestock feed, and increased demand for meat will lead to increased demand for GM crops with which to feed them.[57] Feed grain usage as a percentage of total crop production is 70% for corn and more than 90% of oil seed meals such as soybeans. About 65 million metric tons of GM corn grains and about 70 million metric tons of soybean meals derived from GM soybean are fed to livestock each year.[57]

Uses, actual and proposed[edit]

GM crops grown today, or under experimental development, have been modified with traits intended to provide benefit to farmers, consumers, or industry. These traits include improved shelf life, disease resistance, stress resistance, herbicide resistance, pest resistance, production of useful goods such as biofuel or drugs, and ability to absorb toxins, for use in bioremediation of pollution. Due to high regulatory and research costs, the majority of genetically modified crops in agriculture consist of commodity crops, such as soybean, maize, cotton and rapeseed.[58][59] Recently, some research and development has been targeted to enhancement of crops that are locally important in developing countries, such as insect-resistant cowpea for Africa[60] and insect-resistant brinjal (eggplant) for India.[61]

Improved shelf life[edit]

The first genetically modified crop approved for sale in the U.S. was the FlavrSavr tomato, which had a longer shelf life.[23] It is no longer on the market. As of 2013, an apple that has been genetically modified to resist browning, known as the Nonbrowning Arctic apple produced by Okanagan Specialty Fruits, is awaiting regulatory approval in the US and Canada. A gene in the fruit has been modified such that the apple produces less polyphenol oxidase, a chemical that manifests the browning.[62] If approved by U.S. regulators in the coming months, they will be one of the first genetically engineered fruits on store shelves in America.[63]

Improved nutrition[edit]

Some GM soybeans on the market today offer improved oil profiles for processing or healthier edible oils.[64] [65] GM plants are being developed by both private companies and public research institutions such as CIMMYT, the International Maize and Wheat Improvement Centre.[66] Other examples of GM crops under developmment include a genetically modified cassava with lower cyanogen glucosides and enhanced with protein and other nutrients (called BioCassava, funded by the Bill & Melinda Gates Foundation),[67] while golden rice, developed by the International Rice Research Institute (IRRI), has been discussed as a possible cure for Vitamin A deficiency.[68] An international group of academics has generated a vitamin-enriched corn derived from South African white corn variety M37W with 169x increase in beta carotene, 6x the vitamin C and 2x folate – it is not in production anywhere, but proves that this can be done.[69] Camelina sativa has been modified in research labs to produce plants that accumulate high levels of oils similar to fish oils.[70][71]

Stress resistance[edit]

Plants engineered to tolerate non-biological stresses like drought,[72][73] frost,[74][75] high soil salinity,[76][77] and nitrogen starvation[78] or with increased nutritional value (e.g. Golden rice[79]) were in development in 2011. In 2011, Monsanto's DroughtGard became the first drought resistant GM crop - a genetically modified maize to receive marketing approval in the US.[80]

Herbicide resistance[edit]

One of the most prevalent type of GM crops have a "Roundup Ready", or glyphosate-resistant trait.[32] Tobacco plants have been engineered to be resistant to the herbicide bromoxynil.[24] Crops have been commercialized that are resistant to the herbicide glufosinate, as well.[81] As weeds have grown resistant to glyphosate and other herbicides used in concert with resistant GM crops, companies are developing crops engineered to become resistant to multiple herbicides to allow farmers to use a mixed group of two, three, or four different chemicals.[82][83]

Pathogen resistance[edit]

Tobacco, corn, rice and many other crops, have been generated that express genes encoding for insecticidal proteins from Bacillus thuringiensis (Bt).[25][84] Papaya, potatoes, and squash have been engineered to resist viral pathogens, such as cucumber mosaic virus which, despite its name, infects a wide variety of plants.[85] As of 2013, trials are underway on genetically modified oranges that can resist citrus greening disease.

Production of biofuels[edit]

Algae, both hybrid and GM, is under development by several companies for the production of biofuels.[86] Jatropha has also been modified to improve its qualities for fuel product. Swiss-based Syngenta has received USDA approval to market a maize seed trademarked Enogen, which has been genetically modified to convert its own starch to sugar to speed the process of making ethanol for biofuel.[87] In 2013, the Flemish Institute for Biotechnology was investigating poplar trees genetically engineered to contain less lignin so that they would be more suitable for conversion into biofuels.[88] Lignin is the critical limiting factor when using wood to make bio-ethanol because lignin limits the accessibility of cellulose microfibrils to depolymerization by enzymes.[89]

Production of useful by-products[edit]

Drugs[edit]

In 2012, the FDA approved the first plant-produced pharmaceutical, a treatment for Gaucher's Disease.[90] Tobacco plants have been developed and studied, but are not in production, that can produce therapeutic antibodies.[91]

Antigens[edit]

Bananas have been developed that produce human vaccines against infectious diseases such as Hepatitis B.[92] Another example is the expression of a fusion protein in alfalfa transgenic plants for the selective directioning to antigen presenting cells, therefore increasing vaccine potency against Bovine Viral Diarrhea Virus (BVDV).[93][94]

Materials[edit]

Several companies and labs are working on engineering plants that can be used to make bioplastics.[95] Potatoes that produce more industrially useful starches have been developed as well.[96] Additionally, oilseed can be modified to produce fatty acids for detergents, substitute fuels and petrochemicals.

Bioremediation[edit]

Scientists at the University of York developed a weed (Arabidopsis thaliana) that contains genes from bacteria that can clean up TNT and RDX-explosive contaminants from the soil: It was hoped that this weed would eliminate this pollution.[97] 16 million hectares in the USA (1.5% of the total surface) are estimated to be contaminated with TNT and RDX. However the weed Arabidopsis thaliana was not tough enough to withstand the environment on military test grounds and research is continuing with the University of Washington to develop a tougher native grass.[98]

Genetically modified plants have also been used for bioremediation of contaminated soils. Mercury, selenium and organic pollutants such as polychlorinated biphenyls (PCBs), TNT and RDX explosive contaminants have been removed from soils by transgenic plants containing genes for bacterial enzymes.[98][99]

Marine environments are especially vulnerable since oil spills of coastal regions and the open sea are poorly containable and mitigation is difficult. In addition to pollution through human activities, millions of tons of petroleum enter the marine environment every year from natural seepages. Despite its toxicity, a considerable fraction of petroleum oil entering marine systems is eliminated by the hydrocarbon-degrading activities of microbial communities. Particularly successful is a recently discovered group of specialists, the so-called hydrocarbonoclastic bacteria (HCCB).[100]

Extent of worldwide use of GM crops[edit]

In 2013, GM crops were planted in 27 countries; 19 were developing countries and 8 were developed countries. 2013 was the second year in which developing countries grew a majority (54%) of the total GM harvest. 18 million farmers grew GM crops; around 90% were small-holding farmers in developing countries.[101]

Country 2013– GM planted area (million hectares)[102] Biotech crops
USA 70.1 Maize, Soybean, Cotton, Canola, Sugarbeet, Alfalfa, Papaya, Squash
Brazil 40.3 Soybean, Maize, Cotton
Argentina 24.4 Soybean, Maize, Cotton
India 11.0 Cotton
Canada 10.8 Canola, Maize, Soybean, Sugarbeet
Total 175.2 ----

The United States Department of Agriculture (USDA) reports every year on the total area of GMO varieties planted in the United States.[103][104] According to National Agricultural Statistics Service, the states published in these tables represent 81–86 percent of all corn planted area, 88–90 percent of all soybean planted area, and 81–93 percent of all upland cotton planted area (depending on the year).

Global estimates are produced by the International Service for the Acquisition of Agri-biotech Applications (ISAAA) and can be found in their annual reports, "Global Status of Commercialized Transgenic Crops".[105][101]

Farmers have widely adopted GM technology (see figure). Between 1996 and 2013, the total surface area of land cultivated with GM crops had increased by a factor of 100, from 17,000 square kilometers (4,200,000 acres) to 1,750,000 km2 (432 million acres).[101] 10% of the world's crop lands were planted with GM crops in 2010.[50] As of 2011, 11 different transgenic crops were grown commercially on 395 million acres (160 million hectares) in 29 countries such as the USA, Brazil, Argentina, India, Canada, China, Paraguay, Pakistan, South Africa, Uruguay, Bolivia, Australia, Philippines, Myanmar, Burkina Faso, Mexico and Spain.[50] One of the key reasons for this widespread adoption is the perceived economic benefit the technology brings to farmers. For example, the system of planting glyphosate-resistant seed and then applying glyphosate once plants emerged provided farmers with the opportunity to dramatically increase the yield from a given plot of land, since this allowed them to plant rows closer together.[106] Without it, farmers had to plant rows far enough apart to control post-emergent weeds with mechanical tillage.[106] Likewise, using Bt seeds means that farmers do not have to purchase insecticides, and then invest time, fuel, and equipment in applying them. However critics have disputed whether yields are higher and whether chemical use is less, with GM crops. See Genetically modified food controversies article for information.

Land area used for genetically modified crops by country (1996–2009), in millions of hectares. In 2011, the land area used was 160 million hectares, or 1.6 million square kilometers.[50]

In the US, by 2014, 94% of the planted area of soybeans, 96% of cotton and 93% of corn were genetically modified varieties.[107][108][109] Genetically modified soybeans carried herbicide-tolerant traits only, but maize and cotton carried both herbicide tolerance and insect protection traits (the latter largely the Bacillus thuringiensis Bt insecticidal protein).[110] These constitute "input-traits" which are aimed to financially benefit the producers, but may have indirect environmental benefits and marginal cost benefits to consumers. The Grocery Manufacturers of America estimated in 2003 that 70–75% of all processed foods in the U.S. contained a GM ingredient.[111]

Europe grows relatively few genetically engineered crops[112] with the exception of Spain where one fifth of maize grown is genetically engineered,[113] and smaller amounts in five other countries.[114] The EU had a 'de facto' ban on the approval of new GM crops, from 1999 until 2004;[115] in a controversial move.[116] GM crops are now regulated by the EU.[117] Developing countries grew 54 percent of genetically engineered crops in 2013.[101]

In recent years there has been rapid growth in the area sown in developing countries. A total of 27 countries worldwide grew GM crops in 2013 by approximately 18 million farmers and 54% of GM crops grown worldwide were grown in developing countries.[101] For example, the largest increase in crop area planted to GM crops in 2013 was in Brazil (403,000 km2 versus 368,000 km2 in 2012). There has also been rapid and continuing expansion of GM cotton varieties in India since 2002 with 110,000 km2 of GM cotton harvested in India in 2013.[101] However the use of GM crops in India has been controversial, as discussed in detail in the GM controversies article.

According to the 2013 ISAAA brief: "...a total of 36 countries (35 + EU-28) have granted regulatory approvals for biotech crops for food and/or feed use and for environmental release or planting since 1994... a total of 2,833 regulatory approvals involving 27 GM crops and 336 GM events (NB: an "event" is a specific genetic modification in a specific species) have been issued by competent authorities, of which 1,321 are for food use (direct use or processing), 918 for feed use (direct use or processing) and 599 for environmental release or planting. Japan has the most number of events approved (198), followed by the U.S.A. (165 not including stacked events), Canada (146), Mexico (131), South Korea (103), Australia (93), New Zealand (83), European Union (71 including approvals that have expired or under renewal process), Philippines (68), Taiwan (65), Colombia (59), China (55) and South Africa (52). Maize has the most number of approved events (130 events in 27 countries), followed by cotton (49 events in 22 countries), potato (31 events in 10 countries), canola (30 events in 12 countries) and soybean (27 events in 26 countries).[101]

Examples of genetically modified crops[edit]

Currently, there are a number of food species for which a genetically modified version is being commercially grown (percent modified in the table below are mostly 2009/2010 data).[108][109][118][119][120][121]

Crop Properties of the genetically modified variety Modification[specify] Percent modified in US Percent modified in world
Alfalfa Resistance to glyphosate or glufosinate herbicides New genes added/transferred into plant genome. Planted in the US from 2005–2007; 2007–2010 court injunction; 2011 deregulated
Canola/ Rapeseed Resistance to herbicides (glyphosate or glufosinate), see Roundup Ready Canola high laurate canola,[122] Oleic acid canola[123] New genes added/transferred into plant genome 87% (2005 data[121]) 21%
Corn Resistance to glyphosate or glufosinate herbicides. Insect resistance via producing Bt proteins, some previously used as pesticides in organic crop production. Added enzyme, alpha amylase, that converts starch into sugar to facilitate ethanol production.[124] New genes, some from the bacterium Bacillus thuringiensis, added/transferred into plant genome.[125] 86%[108] 26%
Cotton (cottonseed oil) Kills susceptible insect pests gene for one or more Bt crystal proteins transferred into plant genome 93% 49%
Papaya (Hawaiian) Resistance to the papaya ringspot virus.[126] New gene added/transferred into plant genome 80%
Potato (food) NewLeaf: Bt resistance against Colorado beetle and resistance against Potato virus Y (removed from market in 2001[96]) New Leaf: Bt cry3A, coat protein from PVY[127] 0% 0%
Potato (starch) Amflora: resistance gene against an antibiotic, used for selection, in combination with modifications for better starch production[128] Amflora – antibiotic resistance gene from bacteria; modifications to endogenous starch-producing enzymes 0% 0%
Rice Golden Rice: genetically modified to contain beta-carotene (a source of vitamin A) The version of Golden Rice under development in 2014 contained genes from maize and a common soil microorganism.[129][130] Forecast to be on the market in 2015 or 2016[131]
Soybeans Resistance to glyphosate (see Roundup Ready soybean) or glufosinate herbicides; make less saturated fats;[132] Kills susceptible insect pests Herbicide resistant gene taken from bacteria inserted into soybean; knocked out native genes that catalyze saturation; gene for one or more Bt crystal proteins transferred into plant genome 93% 77%
Squash (Zucchini/Courgette) Resistance to watermelon, cucumber and zucchini/courgette yellow mosaic viruses[123][133][134] Contains coat protein genes of viruses. 13% (figure is from 2005)[121]
Sugar beet Resistance to glyphosate, glufosinate herbicides New genes added/transferred into plant genome 95% (2010); regulated 2011; deregulated 2012 9%
Sugarcane Resistance to certain pesticides, high sucrose content. New genes added/transferred into plant genome
Sweet peppers Resistance to cucumber mosaic virus[135][136] Contains coat protein genes of the virus. Small quantities grown in China
Tomatoes Suppression of the enzyme polygalacturonase (PG), retarding fruit softening after harvesting,[137] while at the same time retaining both the natural color and flavor of the fruit A reverse copy (an antisense gene) of the gene responsible for the production of PG enzyme added into plant genome Taken off the market due to commercial failure. Small quantities grown in China
Wheat Resistance to glyphosate herbicide New genes added/transferred into plant genome unknown unknown

Effects on farming practices[edit]

Managing emergence of resistance[edit]

Constant exposure to a toxin creates evolutionary pressure for pests resistant to that toxin.

One method of reducing resistance is the creation of non-Bt crop refuges to allow some nonresistant insects to survive and maintain a susceptible population. To reduce the chance an insect would become resistant to a Bt crop, the commercialization of transgenic cotton and maize in 1996 was accompanied with a management strategy to prevent insects from becoming resistant to Bt crops, and insect resistance management plans are mandatory for Bt crops planted in the USA and other countries. The aim is to encourage a large population of pests so that any resistance genes that are recessive are greatly diluted within the population.[138]

This means that with sufficiently high levels of transgene expression, nearly all of the heterozygotes (S/s), i.e., the largest segment of the pest population carrying a resistance allele, will be killed before they reach maturity, thus preventing transmission of the resistance gene to their progeny.[139] The planting of refuges (i. e., fields of nontransgenic plants) adjacent to fields of transgenic plants increases the likelihood that homozygous resistant (s/s) individuals and any surviving heterozygotes will mate with susceptible (S/S) individuals from the refuge, instead of with other individuals carrying the resistance allele. As a result, the resistance gene frequency in the population would remain low.

Nevertheless, limitations can affect the success of the high-dose/refuge strategy. For example, expression of the Bt gene can vary. For instance, if the temperature is not ideal, this stress can lower the toxin production and make the plant more susceptible. More importantly, reduced late-season expression of toxin has been documented, possibly resulting from DNA methylation of the promoter.[140] So, while the high-dose/refuge strategy has been successful at prolonging the durability of Bt crops, this success has also had much to do with key factors independent of management strategy, including low initial resistance allele frequencies, fitness costs associated with resistance, and the abundance of non-Bt host plants that have supplemented the refuges planted as part of the resistance management strategy.[141]

Companies that produce Bt seed are addressing this as well, by introducing plants with multiple Bt proteins. Monsanto did this with Bt cotton in India, where the product was rapidly adopted.[142]

Regulation[edit]

The regulation of genetic engineering concerns the approaches taken by governments to assess and manage the risks associated with the development and release of genetically modified crops. There are differences in the regulation of GM crops between countries, with some of the most marked differences occurring between the USA and Europe. Regulation varies in a given country depending on the intended use of the products of the genetic engineering. For example, a crop not intended for food use is generally not reviewed by authorities responsible for food safety.[143][144]

Controversy[edit]

The genetically modified foods controversy is a dispute over the use of food and other goods derived from genetically modified crops instead of from conventional crops, and other uses of genetic engineering in food production. The dispute involves consumers, biotechnology companies, governmental regulators, non-governmental organizations, and scientists. The key areas of controversy related to genetically modified food are: whether GM food should be labeled, the role of government regulators, the effect of GM crops on health and the environment, the effect on pesticide resistance, the impact of GM crops for farmers, and the role of GM crops in feeding the world population.

There is broad scientific consensus that food on the market derived from GM crops poses no greater risk than conventional food.[1][3][145] No reports of ill effects have been documented in the human population from GM food.[4][146][147] Although labeling of genetically modified organism (GMO) products in the marketplace is required in many countries, in the United States, the Food and Drug Administration does not require labeling of GMO products in the marketplace, nor does it recognize a distinction between GMO and non-GMO foods.[148]

Some advocacy groups such as Greenpeace and the World Wildlife Fund have concerns that risks of GM food have not been adequately identified and managed, and have questioned the objectivity of regulatory authorities. Other environmental groups, including The Nature Conservancy[149] and former anti-GMO campaigner Mark Lynas support the use of GMOs as beneficial for the environment.[150]

See also[edit]

References[edit]

  1. ^ a b American Association for the Advancement of Science (AAAS), Board of Directors (2012). Legally Mandating GM Food Labels Could Mislead and Falsely Alarm Consumers
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External links[edit]