Trichoderma reesei
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Trichoderma reesei is a mesophilic and filamentous fungus. It is an anamorph of the fungus Hypocrea jecorina. T. reesei has the capacity to secrete large amounts of cellulolytic enzymes (cellulases and hemicellulases). Microbial cellulases have industrial application in the conversion of cellulose, a major component of plant biomass, into glucose.[1]
Recent advances in the biochemistry of cellulase enzymology, the mechanism of cellulose hydrolysis (cellulolysis), strain improvement, molecular cloning and process engineering are bringing T. reesei cellulases closer to being a commercially viable route to cellulose hydrolysis.[2] Several industrially useful strains have been developed and characterised, e.g. Rut-C30[3], RL-P37 and MCG-80. The genome of this organism was released in 2008.[4]
[edit] See also
- Cellobiohydrolase (CBH)
- Endoglucanase (EG)
- Cellulosic ethanol
[edit] References
- ^ Kumar R, Singh S, Singh OV (May 2008). "Bioconversion of lignocellulosic biomass: biochemical and molecular perspectives". J. Ind. Microbiol. Biotechnol. 35 (5): 377–91. doi:. PMID 18338189.
- ^ Viikari L, Alapuranen M, Puranen T, Vehmaanperä J, Siika-Aho M (2007). "Thermostable enzymes in lignocellulose hydrolysis". Adv. Biochem. Eng. Biotechnol. 108: 121–45. doi:. PMID 17589813.
- ^ [Seidl V, Gamauf C, Druzhinina IS, Seiboth B, Hartl L, Kubicek CP (2008). "The Hypocrea jecorina (Trichoderma reesei) hypercellulolytic mutant RUT C30 lacks a 85 kb (29 gene-encoding) region of the wild-type genome". BMC Genomics 9: 327. doi:. PMID 18620557. PMC: 2483294. http://www.biomedcentral.com/1471-2164/9/327.
- ^ Martinez D, Berka RM, Henrissat B, et al. (May 2008). "Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina)". Nat. Biotechnol. 26 (5): 553–60. doi:. PMID 18454138.
[edit] External links
- http://www.eere.energy.gov/cleancities/progs/afdc/vwbs2.cgi?200
- Trichoderma spp.
- from genencor DNA sequence and Trichoderma reesei EST Database and Mitochondrial Genome.
- Nidetzky B, Steiner W, Claeyssens M (November 1994). "Cellulose hydrolysis by the cellulases from Trichoderma reesei: adsorptions of two cellobiohydrolases, two endocellulases and their core proteins on filter paper and their relation to hydrolysis". Biochem. J. 303 (Pt 3): 817–23. PMID 7980450.
- Miettinen-Oinonen A, Suominen P (August 2002). "Enhanced production of Trichoderma reesei endoglucanases and use of the new cellulase preparations in producing the stonewashed effect on denim fabric". Appl. Environ. Microbiol. 68 (8): 3956–64. PMID 12147496. PMC: 124001. http://aem.asm.org/cgi/content/full/68/8/3956.
- CBH I from Trichoderma sp..
- CBH I from Trichoderma sp..
- Medve J, Ståhlberg J, Tjerneld F (November 1994). "Adsorption and synergism of cellobiohydrolase I and II of Trichoderma reesei during hydrolysis of microcrystalline cellulose". Biotechnol. Bioeng. 44 (9): 1064–73. doi:. PMID 18623023.
This article incorporates public domain material from websites or documents of the United States Department of Energy.
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