Tricine

From Wikipedia, the free encyclopedia
Jump to: navigation, search
Not to be confused with Tricin.
Tricine
Skeletal formula of tricine
Names
IUPAC name
N-(2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)glycine
Other names
Tricine
N-(Tri(hydroxymethyl)methyl)glycine
Identifiers
1937804
5704-04-1 N
ChEBI CHEBI:39063 N
ChemSpider 72078 YesY
EC number 227-193-6
3688
Jmol-3D images Image
Image
MeSH tricine
PubChem 79784
Properties
C6H13NO5
Molar mass 179.17 g·mol−1
Appearance White crystals
89.6 g L−1 (at 20 °C)
UV-vismax) 260 nm
Absorbance 0.03
Related compounds
Related compounds
Milacemide
Except where noted otherwise, data is given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
 N verify (what isYesY/N?)
Infobox references

Tricine is an organic compound that is used in buffer solutions. The name tricine comes from tris and glycine, from which it was derived.[1] It is a white crystalline powder that is moderately soluble in water. It is a zwitterionic amino acid that has a pKa1 value of 2.3 at 25 °C, while its pKa2 at 20 °C is 8.15. Its useful buffering range of pH is 4.4-5.2[citation needed] and 7.4-8.8. Along with bicine, it is one of Good's buffering agents. Good first prepared tricine to buffer chloroplast reactions.

Applications[edit]

Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets. It has a higher negative (more negative) charge than glycine allowing it to migrate faster. In addition its high ionic strength causes more ion movement and less protein movement. This allows for low molecular weight proteins to be separated in lower percent acrylamide gels. Tricine has been documented in the separation of proteins in the range of 1 to 100 kDa by electrophoresis.[2] The tricine buffer at 25 mmol/L was found to be the most effective buffer among the ten tested for ATP assays using firefly luciferase.[3] Tricine has also been found to be an effective scavenger of hydroxyl radicals in a study of radiation-induced membrane damage.[4]

See also[edit]

References[edit]

  1. ^ Good, N.E., et al., Biochemistry, v. 5, 467 (1966).
  2. ^ Schaegger, H., and von Jagow, G., "Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa." "Anal. Biochem." 166(2), 368-379.
  3. ^ Webster, J. J., and Leach, F. R., "Optimization of the firefly luciferase assay for ATP." "J. Appl. Biochem.", 2:469-479.
  4. ^ Hicks, M., and Gebicki, J. M., "Rate constants for reaction of hydroxyl radicals with Tris, Tricine, and Hepes buffers." "FEBS Lett.", 199(1):92-94.