Trypan blue

From Wikipedia, the free encyclopedia
Jump to: navigation, search
Trypan Blue
Trypan blue.png
Identifiers
CAS number 72-57-1 YesY, (tetrasodium salt)
PubChem 5904246
ChemSpider 10482308 YesY
UNII I2ZWO3LS3M YesY
KEGG C19307 N
ChEMBL CHEMBL1640 YesY
Jmol-3D images Image 1
Properties
Molecular formula C34H28N6O14S4
Molar mass 872.88 g mol−1
Melting point > 300
Hazards
LD50 6200 mg/kg (oral, rat)
Except where noted otherwise, data are given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
 N (verify) (what is: YesY/N?)
Infobox references

Trypan blue is a vital stain used to selectively colour dead tissues or cells blue. It is a diazo dye.

Live cells or tissues with intact cell membranes are not coloured. Since cells are very selective in the compounds that pass through the membrane, in a viable cell trypan blue is not absorbed; however, it traverses the membrane in a dead cell. Hence, dead cells are shown as a distinctive blue colour under a microscope. Since live cells are excluded from staining, this staining method is also described as a dye exclusion method. This dye may be a cause of certain birth defects such as encephalocele.

Background & chemistry[edit]

Trypan blue is derived from toluidine, that is, any of several isomeric bases, C14H16N2, derived from toluene. Trypan blue is so-called because it can kill trypanosomes, the parasites that cause sleeping sickness. An analog of trypan blue, suramin is used pharmacologically against trypanosomiasis. Trypan blue is also known as diamine blue and Niagara blue.

The extinction coefficient for trypan blue was found to be 5.17 x 104 M-1 cm-1[1]


Trypan red and Trypan blue were first synthesized by the German scientist Paul Ehrlich in 1904.

Uses of trypan blue[edit]

Observation with an optical microscope of Hyaloperonospora parasitica within a leaf of Arabidopsis thaliana by using the trypan blue staining.

Trypan blue is commonly used in microscopy (for cell counting) and in laboratory mice for assessment of tissue viability. The method cannot distinguish between necrotic and apoptotic cells.

It may be used to observe fungal hyphae[2] and Stramenopiles.

References[edit]

  1. ^ Novel approaches to evaluate osteoarthritis in the rabbit lateral meniscectomy model accessdate=2013-07-08 | pp=33
  2. ^ Nowicki, Marcin et al. (15 May 2013), A simple dual stain for detailed investigations of plant-fungal pathogen interactions, Vegetable Crops Research bulleting, InHort & Versita, doi:10.2478/v10032-012-0016-z, retrieved 2013-05-24 

Wainwright M. Biotech Histochem. 2010 Dec;85(6):341-54. doi: 10.3109/10520290903297528. Dyes, trypanosomiasis and DNA: a historical and critical review.

Trypan blue is also used in Ophtalmic cataract surgery, for staining anterior capsule, in hipermature cataracts, before capsulorhexis.

Synonyms[edit]

  • Azidine Blue 3B
  • Benzamine Blue 3B
  • Benzo Blue 3B
  • Chlorazol Blue 3B
  • Diamine Blue 3B
  • Dianil Blue H3G
  • Direct Blue 14
  • Niagara Blue 3B

External links[edit]