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Tup or TUP may refer to:
- Tup, an uncastrated male sheep
- Tupping, copulation in sheep, or the sheep mating season
- Twopence (disambiguation), two pence
- Tupac Shakur (1971–1996), American rapper and actor
- Technological University of the Philippines
- Telephone User Part, a telephone system protocol used in some parts of the world
- The Urantia Papers, a spiritual and philosophical book
- Tupelo Regional Airport, Tupelo, Mississippi, United States
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