U.S. Route 64 Business was established on May 9, 1956, following the former alignment of US 64. It traverses through downtown Augusta, via 5th Street, Magnolia Street, 3rd Street, and Main Street.
U.S. Route 64 Business starts from US 64 and goes west into downtown Wynne, ending at Falls Boulevard. It does not reconnect to US 64, making it a business spur; not to be confused with US 64 Spur, located northwest of Wynne.
U.S. Route 64 Business (US 64 Bus.) is a 1.07-mile (1.72 km) hidden route through downtown Brevard, via Caldwell Street. Reestablished in late 2006, it reprises the first business loop routing that was decommissioned in 1980. Originally, NCDOT requested AASHTO to split US 64 into US 64E and US 64W, which was denied. The route is not bannered physically as a business route, instead both US 64/US 276 both utilize the routing, with signage directing travelers onto Caldwell Street; however, those that continue on Broad Street will still see signage of US 64/US 276.
Intersection of Meeting Street and Green Street, downtown Morganton
U.S. Route 64 Business (US 64 Bus) was established in 1960, which followed the old mainline US 64 routing through downtown Morganton before 1956, via Burkemont Avenue, Union Street and Meeting Street. Between 1969-1972, US 64 Bus was split into one-way alignments, eastbound continued on Meeting Street while westbound moved onto Union Street. In 1992, a few years after US 64 was rerouted through Lenoir, US 64 Bus was removed east of Green Street along Meeting and Union Streets to Fleming Drive. Eastbound went north on Green Street to Avery Avenue and westbound went south from Sterling Street to Union Street. Continuing along Avery Avenue and Lenoir Road, it reconnects with mainline US 64, via Sanford Drive. This new alignment adds a short overlap with NC 181 and a long overlap with NC 18; its older section west of Green Street continued to share a short overlap with US 70 Bus.
U.S. Route 64 Business was established in 1960, replacing the old mainline US 64 through Wendell and Zebulon. It was extended in 2006 west through Knightdale, reconnecting with mainline US 64 via the Raleigh inner beltway (hidden).
U.S. Route 64 Bypass (US 64 By-pass) was established in 1999 as a new primary routing bypassing Manns Harbor and Manteo, while also providing direct access between Nags Head and the mainland. Its main feature is the Virginia Dare Memorial Bridge, which is a divided four-lane bridge crossing over the Croatan Sound. From 1999-2003, it also shared a complete concurrency with US 264 Bypass.
U.S. Route 64 Spur could be considered a Farm-to-market road, connecting nearby farms to mainline US 64 as oppose to entering the city limits of Wynne. It connects to Martin Luther King Street and John Brown Road. US 64 Spur is a north–south route.
U.S. Route 64 Truck follows US 23 from Franklin to Dillsboro, then east following US 74 to Hendersonville, for a total of 80.8 miles (130.0 km). Signage is not primary, US 64 Truck signs are typically located on the side of major junction changes indicating direction of route. Tractor-trailer trucks are prohibited to use mainline US 64 between Franklin and Brevard.
U.S. Route 64 Alternate (US 64A) was established in 1934 as an alternate route from Bat Cave, in concurrency with US 74, to Ruth, then north to Morganton, replacing NC 181. In 1948 the route became mainline US 64.
U.S. Route 64 Alternate (US 64A) was established in 1934, it replaced the old mainline US 64 through Franklinville and Ramseur. Around 1957, US 64A was downgraded to Andrew Hunter Road and part of NC 22.
U.S. Route 64 Alternate (US 64A) was established in 1934 as an alternate route in downtown Rocky Mount, via Thomas Avenue; while mainline US 64 used Sunset Avenue, Raleigh Street, and Tarboro Street. It was absorbed by mainline US 64 by 1949.
U.S. Route 64 Alternate (US 64A) was established in 1954, it replaced the old mainline US 64 through downtown Williamston, via Main Street, Haughton Street, and Sycamore Street (now Jamesville Road). In 1960 it was renumbered to US 64 Business.
U.S. Route 64 Business was established in 1967, replacing the old mainline US 64 through downtown Enid, via Rock Island Boulevard, Grand Avenue, and Owen K Garriott Road. It was decommissioned in 2009.
U.S. Route 64 Business was established in 1960, as a renumbering of US 64A in downtown Brevard, via Caldwell Street. It was decommissioned by 1980. In 2006, a second US 64 Business was established in Brevard, reprising the original route vacated 26 years earlier.
U.S. Route 64 Business was established in 1968, replacing the old mainline US 64 in Tarboro and Princeville. It was decommissioned either in 1987 (replaced possibility by NC 44) or 1994 (replaced by NC 33).