From Wikipedia, the free encyclopedia
Jump to: navigation, search
U2 small nuclear RNA auxiliary factor 2
Protein U2AF2 PDB 1jmt.png
PDB rendering based on 1jmt.
Available structures
PDB Ortholog search: PDBe, RCSB
Symbols U2AF2 ; U2AF65
External IDs OMIM191318 HomoloGene110853 GeneCards: U2AF2 Gene
RNA expression pattern
PBB GE U2AF2 218381 s at tn.png
PBB GE U2AF2 218382 s at tn.png
More reference expression data
Species Human Mouse
Entrez 11338 22185
Ensembl ENSG00000063244 ENSMUSG00000030435
UniProt P26368 P26369
RefSeq (mRNA) NM_001012478 NM_001205231
RefSeq (protein) NP_001012496 NP_001192160
Location (UCSC) Chr 19:
56.17 – 56.19 Mb
Chr 7:
5.06 – 5.08 Mb
PubMed search [1] [2]

Splicing factor U2AF 65 kDa subunit is a protein that in humans is encoded by the U2AF2 gene.[1]

U2 auxiliary factor (U2AF), composed of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit, which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple alternatively spliced transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.[2]


U2AF2 has been shown to interact with WT1,[3] PUF60,[4][5] SFRS2IP,[6] SRPK2,[4][7] SF1,[4][8][9] U2 small nuclear RNA auxiliary factor 1[4][10] and SFRS11.[11]


  1. ^ Zamore PD, Patton JG, Green MR (Mar 1992). "Cloning and domain structure of the mammalian splicing factor U2AF". Nature 355 (6361): 609–14. doi:10.1038/355609a0. PMID 1538748. 
  2. ^ "Entrez Gene: U2AF2 U2 small nuclear RNA auxiliary factor 2". 
  3. ^ Davies, R C; Calvio C; Bratt E; Larsson S H; Lamond A I; Hastie N D (Oct 1998). "WT1 interacts with the splicing factor U2AF65 in an isoform-dependent manner and can be incorporated into spliceosomes". Genes Dev. (UNITED STATES) 12 (20): 3217–25. doi:10.1101/gad.12.20.3217. ISSN 0890-9369. PMC 317218. PMID 9784496. 
  4. ^ a b c d Rual, Jean-François; Venkatesan Kavitha, Hao Tong, Hirozane-Kishikawa Tomoko, Dricot Amélie, Li Ning, Berriz Gabriel F, Gibbons Francis D, Dreze Matija, Ayivi-Guedehoussou Nono, Klitgord Niels, Simon Christophe, Boxem Mike, Milstein Stuart, Rosenberg Jennifer, Goldberg Debra S, Zhang Lan V, Wong Sharyl L, Franklin Giovanni, Li Siming, Albala Joanna S, Lim Janghoo, Fraughton Carlene, Llamosas Estelle, Cevik Sebiha, Bex Camille, Lamesch Philippe, Sikorski Robert S, Vandenhaute Jean, Zoghbi Huda Y, Smolyar Alex, Bosak Stephanie, Sequerra Reynaldo, Doucette-Stamm Lynn, Cusick Michael E, Hill David E, Roth Frederick P, Vidal Marc (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature (England) 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. 
  5. ^ Ewing, Rob M; Chu Peter, Elisma Fred, Li Hongyan, Taylor Paul, Climie Shane, McBroom-Cerajewski Linda, Robinson Mark D, O'Connor Liam, Li Michael, Taylor Rod, Dharsee Moyez, Ho Yuen, Heilbut Adrian, Moore Lynda, Zhang Shudong, Ornatsky Olga, Bukhman Yury V, Ethier Martin, Sheng Yinglun, Vasilescu Julian, Abu-Farha Mohamed, Lambert Jean-Philippe, Duewel Henry S, Stewart Ian I, Kuehl Bonnie, Hogue Kelly, Colwill Karen, Gladwish Katharine, Muskat Brenda, Kinach Robert, Adams Sally-Lin, Moran Michael F, Morin Gregg B, Topaloglou Thodoros, Figeys Daniel (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. (England) 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. 
  6. ^ Zhang, W J; Wu J Y (Feb 1998). "Sip1, a novel RS domain-containing protein essential for pre-mRNA splicing". Mol. Cell. Biol. (UNITED STATES) 18 (2): 676–84. ISSN 0270-7306. PMC 108778. PMID 9447963. 
  7. ^ Wang, H Y; Lin W; Dyck J A; Yeakley J M; Songyang Z; Cantley L C; Fu X D (Feb 1998). "SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells". J. Cell Biol. (UNITED STATES) 140 (4): 737–50. doi:10.1083/jcb.140.4.737. ISSN 0021-9525. PMC 2141757. PMID 9472028. 
  8. ^ Berglund, J A; Abovich N; Rosbash M (Mar 1998). "A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognition". Genes Dev. (UNITED STATES) 12 (6): 858–67. doi:10.1101/gad.12.6.858. ISSN 0890-9369. PMC 316625. PMID 9512519. 
  9. ^ Abovich, N; Rosbash M (May 1997). "Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals". Cell (UNITED STATES) 89 (3): 403–12. doi:10.1016/S0092-8674(00)80221-4. ISSN 0092-8674. PMID 9150140. 
  10. ^ Zhang, M; Zamore P D; Carmo-Fonseca M; Lamond A I; Green M R (Sep 1992). "Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit". Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 89 (18): 8769–73. doi:10.1073/pnas.89.18.8769. ISSN 0027-8424. PMC 50002. PMID 1388271. 
  11. ^ Zhang, W J; Wu J Y (Oct 1996). "Functional properties of p54, a novel SR protein active in constitutive and alternative splicing". Mol. Cell. Biol. (UNITED STATES) 16 (10): 5400–8. ISSN 0270-7306. PMC 231539. PMID 8816452.