UBE2D1
From Wikipedia, the free encyclopedia
Ubiquitin-conjugating enzyme E2 D1 is a protein that in humans is encoded by the UBE2D1 gene.[1][2][3]
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.[3]
[edit] Interactions
UBE2D1 has been shown to interact with BARD1,[4][5][6][7][8][9][10][11] UBE3A[12][13] and BRCA1.[4][5][6][7][8][9][10][11][14][15]
[edit] References
- ^ Robinson PA, Leek JP, Ardley HC, Thompson J, Rose SA, Markham AF (Mar 1999). "Assignment of UBE2D1 to human chromosome bands 10q11.2→q21 by in situ hybridization". Cytogenet Cell Genet 83 (3–4): 247–8. doi:10.1159/000015195. PMID 10072594.
- ^ Jensen JP, Bates PW, Yang M, Vierstra RD, Weissman AM (Jan 1996). "Identification of a family of closely related human ubiquitin conjugating enzymes". J Biol Chem 270 (51): 30408–14. doi:10.1074/jbc.270.51.30408. PMID 8530467.
- ^ a b "Entrez Gene: UBE2D1 ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast)". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=7321.
- ^ a b Mallery, Donna L; Vandenberg Cassandra J, Hiom Kevin (Dec. 2002). "Activation of the E3 ligase function of the BRCA1/BARD1 complex by polyubiquitin chains". EMBO J. (England) 21 (24): 6755–62. doi:10.1093/emboj/cdf691. ISSN 0261-4189. PMC 139111. PMID 12485996. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139111.
- ^ a b Kentsis, Alex; Gordon Ronald E, Borden Katherine L B (Nov. 2002). "Control of biochemical reactions through supramolecular RING domain self-assembly". Proc. Natl. Acad. Sci. U.S.A. (United States) 99 (24): 15404–9. doi:10.1073/pnas.202608799. ISSN 0027-8424. PMC 137729. PMID 12438698. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=137729.
- ^ a b Chen, Angus; Kleiman Frida E, Manley James L, Ouchi Toru, Pan Zhen-Qiang (Jun. 2002). "Autoubiquitination of the BRCA1*BARD1 RING ubiquitin ligase". J. Biol. Chem. (United States) 277 (24): 22085–92. doi:10.1074/jbc.M201252200. ISSN 0021-9258. PMID 11927591.
- ^ a b Dong, Yuanshu; Hakimi Mohamed-Ali, Chen Xiaowei, Kumaraswamy Easwari, Cooch Neil S, Godwin Andrew K, Shiekhattar Ramin (Nov. 2003). "Regulation of BRCC, a holoenzyme complex containing BRCA1 and BRCA2, by a signalosome-like subunit and its role in DNA repair". Mol. Cell (United States) 12 (5): 1087–99. doi:10.1016/S1097-2765(03)00424-6. ISSN 1097-2765. PMID 14636569.
- ^ a b Sato, Ko; Hayami Ryosuke, Wu Wenwen, Nishikawa Toru, Nishikawa Hiroyuki, Okuda Yoshiko, Ogata Haruki, Fukuda Mamoru, Ohta Tomohiko (Jul. 2004). "Nucleophosmin/B23 is a candidate substrate for the BRCA1-BARD1 ubiquitin ligase". J. Biol. Chem. (United States) 279 (30): 30919–22. doi:10.1074/jbc.C400169200. ISSN 0021-9258. PMID 15184379.
- ^ a b Wu-Baer, Foon; Lagrazon Karen, Yuan Wei, Baer Richard (Sep. 2003). "The BRCA1/BARD1 heterodimer assembles polyubiquitin chains through an unconventional linkage involving lysine residue K6 of ubiquitin". J. Biol. Chem. (United States) 278 (37): 34743–6. doi:10.1074/jbc.C300249200. ISSN 0021-9258. PMID 12890688.
- ^ a b Vandenberg, Cassandra J; Gergely Fanni, Ong Chong Yi, Pace Paul, Mallery Donna L, Hiom Kevin, Patel Ketan J (Jul. 2003). "BRCA1-independent ubiquitination of FANCD2". Mol. Cell (United States) 12 (1): 247–54. doi:10.1016/S1097-2765(03)00281-8. ISSN 1097-2765. PMID 12887909.
- ^ a b Hashizume, R; Fukuda M, Maeda I, Nishikawa H, Oyake D, Yabuki Y, Ogata H, Ohta T (May. 2001). "The RING heterodimer BRCA1-BARD1 is a ubiquitin ligase inactivated by a breast cancer-derived mutation". J. Biol. Chem. (United States) 276 (18): 14537–40. doi:10.1074/jbc.C000881200. ISSN 0021-9258. PMID 11278247.
- ^ Nuber, U; Schwarz S, Kaiser P, Schneider R, Scheffner M (Feb. 1996). "Cloning of human ubiquitin-conjugating enzymes UbcH6 and UbcH7 (E2-F1) and characterization of their interaction with E6-AP and RSP5". J. Biol. Chem. (UNITED STATES) 271 (5): 2795–800. doi:10.1074/jbc.271.5.2795. ISSN 0021-9258. PMID 8576257.
- ^ Nuber, U; Scheffner M (Mar. 1999). "Identification of determinants in E2 ubiquitin-conjugating enzymes required for hect E3 ubiquitin-protein ligase interaction". J. Biol. Chem. (UNITED STATES) 274 (11): 7576–82. doi:10.1074/jbc.274.11.7576. ISSN 0021-9258. PMID 10066826.
- ^ Brzovic, Peter S; Keeffe Jennifer R, Nishikawa Hiroyuki, Miyamoto Keiko, Fox David, Fukuda Mamoru, Ohta Tomohiko, Klevit Rachel (May. 2003). "Binding and recognition in the assembly of an active BRCA1/BARD1 ubiquitin-ligase complex". Proc. Natl. Acad. Sci. U.S.A. (United States) 100 (10): 5646–51. doi:10.1073/pnas.0836054100. ISSN 0027-8424. PMC 156255. PMID 12732733. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=156255.
- ^ Nishikawa, Hiroyuki; Ooka Seido, Sato Ko, Arima Kei, Okamoto Joji, Klevit Rachel E, Fukuda Mamoru, Ohta Tomohiko (Feb. 2004). "Mass spectrometric and mutational analyses reveal Lys-6-linked polyubiquitin chains catalyzed by BRCA1-BARD1 ubiquitin ligase". J. Biol. Chem. (United States) 279 (6): 3916–24. doi:10.1074/jbc.M308540200. ISSN 0021-9258. PMID 14638690.
[edit] Further reading
- Scheffner M, Huibregtse JM, Howley PM (1994). "Identification of a human ubiquitin-conjugating enzyme that mediates the E6-AP-dependent ubiquitination of p53". Proc. Natl. Acad. Sci. U.S.A. 91 (19): 8797–801. doi:10.1073/pnas.91.19.8797. PMC 44693. PMID 8090726. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=44693.
- Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
- Hatakeyama S, Jensen JP, Weissman AM (1997). "Subcellular localization and ubiquitin-conjugating enzyme (E2) interactions of mammalian HECT family ubiquitin protein ligases". J. Biol. Chem. 272 (24): 15085–92. doi:10.1074/jbc.272.24.15085. PMID 9182527.
- Gutierrez JA, Yu J, Rivera S, Wessling-Resnick M (1997). "Functional Expression Cloning and Characterization of SFT, a Stimulator of Fe Transport". J. Cell Biol. 139 (4): 895–905. doi:10.1083/jcb.139.4.895. PMC 2139974. PMID 9362508. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2139974.
- Gutierrez JA, Yu J, Wessling-Resnick M (1999). "Characterization and chromosomal mapping of the human gene for SFT, a stimulator of Fe transport". Biochem. Biophys. Res. Commun. 253 (3): 739–42. doi:10.1006/bbrc.1998.9836. PMID 9918797.
- Nuber U, Scheffner M (1999). "Identification of determinants in E2 ubiquitin-conjugating enzymes required for hect E3 ubiquitin-protein ligase interaction". J. Biol. Chem. 274 (11): 7576–82. doi:10.1074/jbc.274.11.7576. PMID 10066826.
- Kamura T, Sato S, Iwai K, et al. (2000). "Activation of HIF1α ubiquitination by a reconstituted von Hippel-Lindau (VHL) tumor suppressor complex". Proc. Natl. Acad. Sci. U.S.A. 97 (19): 10430–5. doi:10.1073/pnas.190332597. PMC 27041. PMID 10973499. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=27041.
- Pringa E, Martinez-Noel G, Muller U, Harbers K (2001). "Interaction of the ring finger-related U-box motif of a nuclear dot protein with ubiquitin-conjugating enzymes". J. Biol. Chem. 276 (22): 19617–23. doi:10.1074/jbc.M100192200. PMID 11274149.
- Hashizume R, Fukuda M, Maeda I, et al. (2001). "The RING heterodimer BRCA1-BARD1 is a ubiquitin ligase inactivated by a breast cancer-derived mutation". J. Biol. Chem. 276 (18): 14537–40. doi:10.1074/jbc.C000881200. PMID 11278247.
- Matsuzawa SI, Reed JC (2001). "Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses". Mol. Cell 7 (5): 915–26. doi:10.1016/S1097-2765(01)00242-8. PMID 11389839.
- Jiang J, Ballinger CA, Wu Y, et al. (2001). "CHIP is a U-box-dependent E3 ubiquitin ligase: identification of Hsc70 as a target for ubiquitylation". J. Biol. Chem. 276 (46): 42938–44. doi:10.1074/jbc.M101968200. PMID 11557750.
- Chen A, Kleiman FE, Manley JL, et al. (2002). "Autoubiquitination of the BRCA1*BARD1 RING ubiquitin ligase". J. Biol. Chem. 277 (24): 22085–92. doi:10.1074/jbc.M201252200. PMID 11927591.
- Badciong JC, Haas AL (2003). "MdmX is a RING finger ubiquitin ligase capable of synergistically enhancing Mdm2 ubiquitination". J. Biol. Chem. 277 (51): 49668–75. doi:10.1074/jbc.M208593200. PMID 12393902.
- Kentsis A, Gordon RE, Borden KL (2003). "Control of biochemical reactions through supramolecular RING domain self-assembly". Proc. Natl. Acad. Sci. U.S.A. 99 (24): 15404–9. doi:10.1073/pnas.202608799. PMC 137729. PMID 12438698. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=137729.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
- Gehrke SG, Riedel HD, Herrmann T, et al. (2003). "UbcH5A, a member of human E2 ubiquitin-conjugating enzymes, is closely related to SFT, a stimulator of iron transport, and is up-regulated in hereditary hemochromatosis". Blood 101 (8): 3288–93. doi:10.1182/blood-2002-07-2192. PMID 12480712.
- Mallery DL, Vandenberg CJ, Hiom K (2004). "Activation of the E3 ligase function of the BRCA1/BARD1 complex by polyubiquitin chains". EMBO J. 21 (24): 6755–62. doi:10.1093/emboj/cdf691. PMC 139111. PMID 12485996. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139111.
- Takeyama K, Aguiar RC, Gu L, et al. (2003). "The BAL-binding protein BBAP and related Deltex family members exhibit ubiquitin-protein isopeptide ligase activity". J. Biol. Chem. 278 (24): 21930–7. doi:10.1074/jbc.M301157200. PMID 12670957.
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PDB gallery
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2c4p: CRYSTAL STRUCTURE OF HUMAN UBIQUITIN-CONJUGATING ENZYME UBCH5A
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Chaperones/
protein folding |
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Hsp10/GroES (Early pregnancy factor) · Hsp27 · Hsp47 · HSP60/GroEL
Hsp40/DnaJ (A1, A2, A3, B1, B2, B11, B4, B6, B9, C1, C3, C5, C6, C7, C10, C11, C13, C14, C19)
Hsp70 (1A, 1B, 1L, 2, 4, 4L, 5, 6, 7, 8, 9, 12A, 14)
Hsp90 ( α1, α2, β, ER, TRAP1)
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Other
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| Protein targeting |
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| Ubiquitin |
E1 Ubiquitin-activating enzyme (UBA1, UBA2, UBA3, UBA5, UBA6, UBA7, ATG7, NAE1, SAE1)
E2 Ubiquitin-conjugating enzyme (A • B • C • D1, D2, D3 • E1, E2, E3 • G1, G2 • H • I • J1, J2 • K • L1, L2, L3, L4, L6 • M • N • O • Q1, Q2 • R1 (CDC34), R2 • S • V1, V2 • Z)
E3 Ubiquitin ligase (VHL, Cullin, CBL, MDM2, FANCL, UBR1)
Deubiquitinating enzyme: Ataxin 3 • USP6 • CYLD
ATG3 • BIRC6 • UFC1
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see also posttranslational modification disorders
B bsyn: dna (repl, cycl, reco, repr) · tscr (fact, tcrg, nucl, rnat, rept, ptts) · tltn (risu, pttl, nexn) · dnab, rnab/runp · stru (domn, 1°, 2°, 3°, 4°)
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