UBE2I

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Ubiquitin-conjugating enzyme E2I
Protein UBE2I PDB 1a3s.png
PDB rendering based on 1a3s.
Available structures
PDB Ortholog search: PDBe, RCSB
Identifiers
Symbols UBE2I ; C358B7.1; P18; UBC9
External IDs OMIM601661 MGI107365 HomoloGene5574 ChEMBL: 1741191 GeneCards: UBE2I Gene
EC number 6.3.2.19
RNA expression pattern
PBB GE UBE2I 213535 s at tn.png
PBB GE UBE2I 208760 at tn.png
PBB GE UBE2I 211008 s at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez 7329 22196
Ensembl ENSG00000103275 ENSMUSG00000015120
UniProt P63279 P63280
RefSeq (mRNA) NM_003345 NM_001177609
RefSeq (protein) NP_003336 NP_001171080
Location (UCSC) Chr 16:
1.36 – 1.38 Mb
Chr 17:
25.26 – 25.27 Mb
PubMed search [1] [2]

SUMO-conjugating enzyme UBC9 is a protein that in humans is encoded by the UBE2I gene.[1]

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.[2]

Interactions[edit]

UBE2I has been shown to interact with WT1,[3] TOP1,[4] RAD51,[5][6] Activating transcription factor 2,[7] PIAS1,[8][9][10] SALL1,[11] FHIT,[12] RANBP2,[13][14] Protein inhibitor of activated STAT2,[8][15] DNMT3A,[16] DNMT3B,[17] Small ubiquitin-related modifier 1,[6][13][18][19] SAE2,[13][20] RANGAP1,[13][18][20] Death associated protein 6,[20][21] Androgen receptor,[22] ETS1,[23] IPO13,[24] TCF3,[25] TNFRSF1A,[26] DACH1,[15][27] P53,[6][19][28][29] BLMH,[30] MAP3K1[26] and Microphthalmia-associated transcription factor.[31]

Notes[edit]

  1. ^ Watanabe TK, Fujiwara T, Kawai A, Shimizu F, Takami S, Hirano H, Okuno S, Ozaki K, Takeda S, Shimada Y, Nagata M, Takaichi A, Takahashi E, Nakamura Y, Shin S (March 1996). "Cloning, expression, and mapping of UBE2I, a novel gene encoding a human homologue of yeast ubiquitin-conjugating enzymes which are critical for regulating the cell cycle". Cytogenet Cell Genet 72 (1): 86–9. doi:10.1159/000134169. PMID 8565643. 
  2. ^ "Entrez Gene: UBE2I ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)". 
  3. ^ Wang, Z Y; Qiu Q Q; Seufert W; Taguchi T; Testa J R; Whitmore S A; Callen D F; Welsh D; Shenk T; Deuel T F (October 1996). "Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9". J. Biol. Chem. (UNITED STATES) 271 (40): 24811–6. doi:10.1074/jbc.271.40.24811. ISSN 0021-9258. PMID 8798754. 
  4. ^ Mao, Y; Sun M; Desai S D; Liu L F (April 2000). "SUMO-1 conjugation to topoisomerase I: A possible repair response to topoisomerase-mediated DNA damage". Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 97 (8): 4046–51. doi:10.1073/pnas.080536597. ISSN 0027-8424. PMC 18143. PMID 10759568. 
  5. ^ Kovalenko, O V; Plug A W; Haaf T; Gonda D K; Ashley T; Ward D C; Radding C M; Golub E I (April 1996). "Mammalian ubiquitin-conjugating enzyme Ubc9 interacts with Rad51 recombination protein and localizes in synaptonemal complexes". Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 93 (7): 2958–63. doi:10.1073/pnas.93.7.2958. ISSN 0027-8424. PMC 39742. PMID 8610150. 
  6. ^ a b c Shen, Z; Pardington-Purtymun P E; Comeaux J C; Moyzis R K; Chen D J (October 1996). "Associations of UBE2I with RAD52, UBL1, p53, and RAD51 proteins in a yeast two-hybrid system". Genomics (UNITED STATES) 37 (2): 183–6. doi:10.1006/geno.1996.0540. ISSN 0888-7543. PMID 8921390. 
  7. ^ Firestein, R; Feuerstein N (March 1998). "Association of activating transcription factor 2 (ATF2) with the ubiquitin-conjugating enzyme hUBC9. Implication of the ubiquitin/proteasome pathway in regulation of ATF2 in T cells". J. Biol. Chem. (UNITED STATES) 273 (10): 5892–902. doi:10.1074/jbc.273.10.5892. ISSN 0021-9258. PMID 9488727. 
  8. ^ a b Lee, Byoung-Hee; Yoshimatsu Kumiko; Maeda Akihiko; Ochiai Kazuhiko; Morimatsu Masami; Araki Koichi; Ogino Michiko; Morikawa Shigeru; Arikawa Jiro (Dec 2003). "Association of the nucleocapsid protein of the Seoul and Hantaan hantaviruses with small ubiquitin-like modifier-1-related molecules". Virus Res. (Netherlands) 98 (1): 83–91. doi:10.1016/j.virusres.2003.09.001. ISSN 0168-1702. PMID 14609633. 
  9. ^ Sapetschnig, Alexandra; Rischitor Grigore; Braun Harald; Doll Andreas; Schergaut Marion; Melchior Frauke; Suske Guntram (October 2002). "Transcription factor Sp3 is silenced through SUMO modification by PIAS1". EMBO J. (England) 21 (19): 5206–15. doi:10.1093/emboj/cdf510. ISSN 0261-4189. PMC 129032. PMID 12356736. 
  10. ^ Kahyo, T; Nishida T; Yasuda H (September 2001). "Involvement of PIAS1 in the sumoylation of tumor suppressor p53". Mol. Cell (United States) 8 (3): 713–8. doi:10.1016/S1097-2765(01)00349-5. ISSN 1097-2765. PMID 11583632. 
  11. ^ Netzer, Christian; Bohlander Stefan K; Rieger Leonie; Müller Stefan; Kohlhase Jürgen (August 2002). "Interaction of the developmental regulator SALL1 with UBE2I and SUMO-1". Biochem. Biophys. Res. Commun. (United States) 296 (4): 870–6. doi:10.1016/S0006-291X(02)02003-X. ISSN 0006-291X. PMID 12200128. 
  12. ^ Shi, Y; Zou M; Farid N R; Paterson M C (Dec 2000). "Association of FHIT (fragile histidine triad), a candidate tumour suppressor gene, with the ubiquitin-conjugating enzyme hUBC9". Biochem. J. (ENGLAND). 352 Pt 2 (Pt 2): 443–8. doi:10.1042/0264-6021:3520443. ISSN 0264-6021. PMC 1221476. PMID 11085938. 
  13. ^ a b c d Ewing, Rob M; Chu Peter, Elisma Fred, Li Hongyan, Taylor Paul, Climie Shane, McBroom-Cerajewski Linda, Robinson Mark D, O'Connor Liam, Li Michael, Taylor Rod, Dharsee Moyez, Ho Yuen, Heilbut Adrian, Moore Lynda, Zhang Shudong, Ornatsky Olga, Bukhman Yury V, Ethier Martin, Sheng Yinglun, Vasilescu Julian, Abu-Farha Mohamed, Lambert Jean-Philippe, Duewel Henry S, Stewart Ian I, Kuehl Bonnie, Hogue Kelly, Colwill Karen, Gladwish Katharine, Muskat Brenda, Kinach Robert, Adams Sally-Lin, Moran Michael F, Morin Gregg B, Topaloglou Thodoros, Figeys Daniel (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. (England) 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. 
  14. ^ Zhang, Hong; Saitoh Hisato; Matunis Michael J (September 2002). "Enzymes of the SUMO Modification Pathway Localize to Filaments of the Nuclear Pore Complex". Mol. Cell. Biol. (United States) 22 (18): 6498–508. doi:10.1128/MCB.22.18.6498-6508.2002. ISSN 0270-7306. PMC 135644. PMID 12192048. 
  15. ^ a b Rual, Jean-François; Venkatesan Kavitha, Hao Tong, Hirozane-Kishikawa Tomoko, Dricot Amélie, Li Ning, Berriz Gabriel F, Gibbons Francis D, Dreze Matija, Ayivi-Guedehoussou Nono, Klitgord Niels, Simon Christophe, Boxem Mike, Milstein Stuart, Rosenberg Jennifer, Goldberg Debra S, Zhang Lan V, Wong Sharyl L, Franklin Giovanni, Li Siming, Albala Joanna S, Lim Janghoo, Fraughton Carlene, Llamosas Estelle, Cevik Sebiha, Bex Camille, Lamesch Philippe, Sikorski Robert S, Vandenhaute Jean, Zoghbi Huda Y, Smolyar Alex, Bosak Stephanie, Sequerra Reynaldo, Doucette-Stamm Lynn, Cusick Michael E, Hill David E, Roth Frederick P, Vidal Marc (October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature (England) 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. 
  16. ^ Ling, Yan; Sankpal Umesh T; Robertson Andrea K; McNally James G; Karpova Tatiana; Robertson Keith D (2004). "Modification of de novo DNA methyltransferase 3a (Dnmt3a) by SUMO-1 modulates its interaction with histone deacetylases (HDACs) and its capacity to repress transcription". Nucleic Acids Res. (England) 32 (2): 598–610. doi:10.1093/nar/gkh195. PMC 373322. PMID 14752048. 
  17. ^ Kang, E S; Park C W; Chung J H (Dec 2001). "Dnmt3b, de novo DNA methyltransferase, interacts with SUMO-1 and Ubc9 through its N-terminal region and is subject to modification by SUMO-1". Biochem. Biophys. Res. Commun. (United States) 289 (4): 862–8. doi:10.1006/bbrc.2001.6057. ISSN 0006-291X. PMID 11735126. 
  18. ^ a b Tatham, Michael H; Kim Suhkmann; Yu Bin; Jaffray Ellis; Song Jing; Zheng Jian; Rodriguez Manuel S; Hay Ronald T; Chen Yuan (August 2003). "Role of an N-terminal site of Ubc9 in SUMO-1, -2, and -3 binding and conjugation". Biochemistry (United States) 42 (33): 9959–69. doi:10.1021/bi0345283. ISSN 0006-2960. PMID 12924945. 
  19. ^ a b Minty, A; Dumont X; Kaghad M; Caput D (November 2000). "Covalent modification of p73alpha by SUMO-1. Two-hybrid screening with p73 identifies novel SUMO-1-interacting proteins and a SUMO-1 interaction motif". J. Biol. Chem. (UNITED STATES) 275 (46): 36316–23. doi:10.1074/jbc.M004293200. ISSN 0021-9258. PMID 10961991. 
  20. ^ a b c Knipscheer, Puck; Flotho Annette; Klug Helene; Olsen Jesper V; van Dijk Willem J; Fish Alexander; Johnson Erica S; Mann Matthias; Sixma Titia K; Pichler Andrea (August 2008). "Ubc9 sumoylation regulates SUMO target discrimination". Mol. Cell (United States) 31 (3): 371–82. doi:10.1016/j.molcel.2008.05.022. PMID 18691969. 
  21. ^ Ryu, S W; Chae S K; Kim E (Dec 2000). "Interaction of Daxx, a Fas binding protein, with sentrin and Ubc9". Biochem. Biophys. Res. Commun. (UNITED STATES) 279 (1): 6–10. doi:10.1006/bbrc.2000.3882. ISSN 0006-291X. PMID 11112409. 
  22. ^ Poukka, H; Aarnisalo P; Karvonen U; Palvimo J J; Jänne O A (July 1999). "Ubc9 interacts with the androgen receptor and activates receptor-dependent transcription". J. Biol. Chem. (UNITED STATES) 274 (27): 19441–6. doi:10.1074/jbc.274.27.19441. ISSN 0021-9258. PMID 10383460. 
  23. ^ Hahn, S L; Wasylyk B; Criqui-Filipe P; Criqui P (September 1997). "Modulation of ETS-1 transcriptional activity by huUBC9, a ubiquitin-conjugating enzyme". Oncogene (ENGLAND) 15 (12): 1489–95. doi:10.1038/sj.onc.1201301. ISSN 0950-9232. PMID 9333025. 
  24. ^ Mingot, J M; Kostka S; Kraft R; Hartmann E; Görlich D (July 2001). "Importin 13: a novel mediator of nuclear import and export". EMBO J. (England) 20 (14): 3685–94. doi:10.1093/emboj/20.14.3685. ISSN 0261-4189. PMC 125545. PMID 11447110. 
  25. ^ Huggins, G S; Chin M T; Sibinga N E; Lee S L; Haber E; Lee M E (October 1999). "Characterization of the mUBC9-binding sites required for E2A protein degradation". J. Biol. Chem. (UNITED STATES) 274 (40): 28690–6. doi:10.1074/jbc.274.40.28690. ISSN 0021-9258. PMID 10497239. 
  26. ^ a b Saltzman, A; Searfoss G, Marcireau C, Stone M, Ressner R, Munro R, Franks C, D'Alonzo J, Tocque B, Jaye M, Ivashchenko Y (April 1998). "hUBC9 associates with MEKK1 and type I TNF-alpha receptor and stimulates NFkappaB activity". FEBS Lett. (NETHERLANDS) 425 (3): 431–5. doi:10.1016/S0014-5793(98)00287-7. ISSN 0014-5793. PMID 9563508. 
  27. ^ Machon, O; Backman M; Julin K; Krauss S (October 2000). "Yeast two-hybrid system identifies the ubiquitin-conjugating enzyme mUbc9 as a potential partner of mouse Dac". Mech. Dev. (IRELAND) 97 (1–2): 3–12. doi:10.1016/S0925-4773(00)00402-0. ISSN 0925-4773. PMID 11025202. 
  28. ^ Gallagher, W M; Argentini M; Sierra V; Bracco L; Debussche L; Conseiller E (June 1999). "MBP1: a novel mutant p53-specific protein partner with oncogenic properties". Oncogene (ENGLAND) 18 (24): 3608–16. doi:10.1038/sj.onc.1202937. ISSN 0950-9232. PMID 10380882. 
  29. ^ Bernier-Villamor, Victor; Sampson Deborah A; Matunis Michael J; Lima Christopher D (February 2002). "Structural basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin-conjugating enzyme Ubc9 and RanGAP1". Cell (United States) 108 (3): 345–56. doi:10.1016/S0092-8674(02)00630-X. ISSN 0092-8674. PMID 11853669. 
  30. ^ Koldamova, R P; Lefterov I M; DiSabella M T; Lazo J S (Dec 1998). "An evolutionarily conserved cysteine protease, human bleomycin hydrolase, binds to the human homologue of ubiquitin-conjugating enzyme 9". Mol. Pharmacol. (UNITED STATES) 54 (6): 954–61. ISSN 0026-895X. PMID 9855622. 
  31. ^ Xu, W; Gong L; Haddad M M; Bischof O; Campisi J; Yeh E T; Medrano E E (March 2000). "Regulation of microphthalmia-associated transcription factor MITF protein levels by association with the ubiquitin-conjugating enzyme hUBC9". Exp. Cell Res. (UNITED STATES) 255 (2): 135–43. doi:10.1006/excr.2000.4803. ISSN 0014-4827. PMID 10694430. 

References[edit]