The 2000–01 UEFA Champions League was the 46th season of the UEFA Champions League, UEFA's premier European club football tournament, and the ninth since it was rebranded from the "European Champion Clubs' Cup" or "European Cup". The competition was won by Bayern Munich (first title since 1976), who beat Valencia 5–4 on penalties after a 1–1 draw after extra time. It was their first UEFA Champions League title, and their fourth European Cup title overall, it was Valencia's second consecutive final defeat, losing to Real Madrid in the previous season. The knockout phase saw Bayern eliminate the preceding two Champions League winners, Manchester United and Real Madrid, winning all four games in the process. Valencia, meanwhile, defeated English sides Arsenal and Leeds United in the knockout phase en route to the final.
The 2001 final saw the two previous seasons losing finalists clash, Bayern Munich lost to Manchester United in the 1999 final and Valencia lost to Real Madrid in the 2000 final.
Real Madrid were the defending champions, but were eliminated by eventual winners Bayern Munich in the semi-finals.
Location of teams of the 2000–01 UEFA Champions League first group stage. Brown: Group A; Red: Group B; Orange: Group C; Yellow: Group D; Green: Group E; Blue: Group F; Purple: Group G; Pink: Group H.
16 winners from the third qualifying round, 10 champions from countries ranked 1–10, and six second-placed teams from countries ranked 1–6 were drawn into eight groups of four teams each. The top two teams in each group advanced to the second group stage, and the third placed team in each group advanced to round 3 of the 2000–01 UEFA Cup.
Eight winners and eight runners up from the first group stage were drawn into 4 groups of 4 teams each, each containing two group winners and two runners-up. Teams from the same country or from the same first round group cannot be drawn together. The top 2 teams in each group advance to the quarter finals.