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UFI may refer to:
- UFI, Union des Foires Internationales, a trade show industry association
- Uniform Floppy Interface, a USB encapsulation protocol for floppy disk drives
- Unión del Fútbol del Interior, the Paraguayan football (soccer) governing body
- Unique Feature Identifier, a globally unique geocode identification for geographical locations and features
- United Families International, a nonprofit organization in Gilbert, Arizona
- Ufi Ltd, a not-for-profit organisation created in 1998 to take forward the UK Government's vision of a University for Industry
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