VAMP2

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Vesicle-associated membrane protein 2 (synaptobrevin 2)
Protein VAMP2 PDB 1kil.png
PDB rendering based on 1kil.
Available structures
PDB Ortholog search: PDBe, RCSB
Identifiers
Symbols VAMP2 ; SYB2; VAMP-2
External IDs OMIM185881 MGI1313277 HomoloGene7591 GeneCards: VAMP2 Gene
RNA expression pattern
PBB GE VAMP2 201556 s at tn.png
PBB GE VAMP2 201557 at tn.png
PBB GE VAMP2 214792 x at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez 6844 22318
Ensembl ENSG00000220205 ENSMUSG00000020894
UniProt P63027 P63044
RefSeq (mRNA) NM_014232 NM_009497
RefSeq (protein) NP_055047 NP_033523
Location (UCSC) Chr 17:
8.06 – 8.07 Mb
Chr 11:
69.09 – 69.09 Mb
PubMed search [1] [2]

Vesicle-associated membrane protein 2 is a protein that in humans is encoded by the VAMP2 gene.[1][2]

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP2 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP2 is thought to participate in neurotransmitter release at a step between docking and fusion. Mice lacking functional synaptobrevin2/VAMP2 gene cannot survive after birth, and have a dramatically reduced synaptic transmission, around 10% of control.[3] The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and complexin. It also forms a distinct complex with synaptophysin.[2]

VAMP2 is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG.

Interactions[edit]

VAMP2 has been shown to interact with STX4,[4][5][6][7] SNAP-25,[8][9][10] SNAP23,[4][11][12] STX1A[9][10][13][14][15][16] and RABAC1.[17]

References[edit]

  1. ^ Archer BT 3rd, Ozcelik T, Jahn R, Francke U, Sudhof TC (November 1990). "Structures and chromosomal localizations of two human genes encoding synaptobrevins 1 and 2". J Biol Chem 265 (28): 17267–73. PMID 1976629. 
  2. ^ a b "Entrez Gene: VAMP2 vesicle-associated membrane protein 2 (synaptobrevin 2)". 
  3. ^ Schoch S, Deák F, Königstorfer A, Mozhayeva M, Sara Y, Südhof TC, Kavalali ET. (November 2001). "SNARE function analyzed in synaptobrevin/VAMP knockout mice". Science 294 (5544): 1117–22. doi:10.1126/science.1064335. PMID 11691998. 
  4. ^ a b Paumet, F; Le Mao J, Martin S, Galli T, David B, Blank U, Roa M (June 2000). "Soluble NSF attachment protein receptors (SNAREs) in RBL-2H3 mast cells: functional role of syntaxin 4 in exocytosis and identification of a vesicle-associated membrane protein 8-containing secretory compartment". J. Immunol. (UNITED STATES) 164 (11): 5850–7. doi:10.4049/jimmunol.164.11.5850. ISSN 0022-1767. PMID 10820264. 
  5. ^ Mollinedo, Faustino; Martín-Martín Belén, Calafat Jero, Nabokina Svetlana M, Lazo Pedro A (January 2003). "Role of vesicle-associated membrane protein-2, through Q-soluble N-ethylmaleimide-sensitive factor attachment protein receptor/R-soluble N-ethylmaleimide-sensitive factor attachment protein receptor interaction, in the exocytosis of specific and tertiary granules of human neutrophils". J. Immunol. (United States) 170 (2): 1034–42. doi:10.4049/jimmunol.170.2.1034. ISSN 0022-1767. PMID 12517971. 
  6. ^ Jagadish, M N; Fernandez C S, Hewish D R, Macaulay S L, Gough K H, Grusovin J, Verkuylen A, Cosgrove L, Alafaci A, Frenkel M J, Ward C W (August 1996). "Insulin-responsive tissues contain the core complex protein SNAP-25 (synaptosomal-associated protein 25) A and B isoforms in addition to syntaxin 4 and synaptobrevins 1 and 2". Biochem. J. 317 (ENGLAND). ( Pt 3): 945–54. ISSN 0264-6021. PMC 1217577. PMID 8760387. 
  7. ^ Reed, G L; Houng A K, Fitzgerald M L (April 1999). "Human platelets contain SNARE proteins and a Sec1p homologue that interacts with syntaxin 4 and is phosphorylated after thrombin activation: implications for platelet secretion". Blood (UNITED STATES) 93 (8): 2617–26. ISSN 0006-4971. PMID 10194441. 
  8. ^ Li, Y; Chin L S, Weigel C, Li L (November 2001). "Spring, a novel RING finger protein that regulates synaptic vesicle exocytosis". J. Biol. Chem. (United States) 276 (44): 40824–33. doi:10.1074/jbc.M106141200. ISSN 0021-9258. PMID 11524423. 
  9. ^ a b Hao, J C; Salem N, Peng X R, Kelly R B, Bennett M K (March 1997). "Effect of mutations in vesicle-associated membrane protein (VAMP) on the assembly of multimeric protein complexes". J. Neurosci. (UNITED STATES) 17 (5): 1596–603. ISSN 0270-6474. PMID 9030619. 
  10. ^ a b Chen, Xiaocheng; Tomchick Diana R, Kovrigin Evguenii, Araç Demet, Machius Mischa, Südhof Thomas C, Rizo Josep (January 2002). "Three-dimensional structure of the complexin/SNARE complex". Neuron (United States) 33 (3): 397–409. doi:10.1016/S0896-6273(02)00583-4. ISSN 0896-6273. PMID 11832227. 
  11. ^ Imai, Akane; Nashida Tomoko, Yoshie Sumio, Shimomura Hiromi (August 2003). "Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE complexes on the apical plasma membrane". Arch. Oral Biol. (England) 48 (8): 597–604. doi:10.1016/S0003-9969(03)00116-X. ISSN 0003-9969. PMID 12828989. 
  12. ^ Kawanishi, M; Tamori Y, Okazawa H, Araki S, Shinoda H, Kasuga M (March 2000). "Role of SNAP23 in insulin-induced translocation of GLUT4 in 3T3-L1 adipocytes. Mediation of complex formation between syntaxin4 and VAMP2". J. Biol. Chem. (UNITED STATES) 275 (11): 8240–7. doi:10.1074/jbc.275.11.8240. ISSN 0021-9258. PMID 10713150. 
  13. ^ Dulubova, I; Sugita S, Hill S, Hosaka M, Fernandez I, Südhof T C, Rizo J (August 1999). "A conformational switch in syntaxin during exocytosis: role of munc18". EMBO J. (ENGLAND) 18 (16): 4372–82. doi:10.1093/emboj/18.16.4372. ISSN 0261-4189. PMC 1171512. PMID 10449403. 
  14. ^ McMahon, H T; Missler M, Li C, Südhof T C (October 1995). "Complexins: cytosolic proteins that regulate SNAP receptor function". Cell (UNITED STATES) 83 (1): 111–9. doi:10.1016/0092-8674(95)90239-2. ISSN 0092-8674. PMID 7553862. 
  15. ^ Pérez-Brangulí, Francesc; Muhaisen Ashraf, Blasi Juan (June 2002). "Munc 18a binding to syntaxin 1A and 1B isoforms defines its localization at the plasma membrane and blocks SNARE assembly in a three-hybrid system assay". Mol. Cell. Neurosci. (United States) 20 (2): 169–80. doi:10.1006/mcne.2002.1122. ISSN 1044-7431. PMID 12093152. 
  16. ^ Margittai, M; Otto H, Jahn R (March 1999). "A stable interaction between syntaxin 1a and synaptobrevin 2 mediated by their transmembrane domains". FEBS Lett. (NETHERLANDS) 446 (1): 40–4. doi:10.1016/S0014-5793(99)00028-9. ISSN 0014-5793. PMID 10100611. 
  17. ^ Martincic, I; Peralta M E, Ngsee J K (October 1997). "Isolation and characterization of a dual prenylated Rab and VAMP2 receptor". J. Biol. Chem. (UNITED STATES) 272 (43): 26991–8. doi:10.1074/jbc.272.43.26991. ISSN 0021-9258. PMID 9341137. 

Further reading[edit]