Vesicle-associated membrane protein 8

Vesicle-associated membrane protein 8 (endobrevin)
PDB rendering based on 1gl2.
Available structures PDB 1gl2
Identifiers
Symbols	VAMP8; EDB; VAMP-8
External IDs	OMIM: 603177 MGI: 1336882 HomoloGene: 37846 GeneCards: VAMP8 Gene
Gene Ontology Molecular function • protein binding Cellular component • membrane fraction • mitochondrion • early endosome • plasma membrane • integral to membrane • secretory granule membrane • SNARE complex Biological process • protein complex assembly • post-Golgi vesicle-mediated transport • cellular membrane fusion • cellular membrane organization • vesicle-mediated transport Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	8673	22320
Ensembl	ENSG00000118640	ENSMUSG00000050732
UniProt	Q9BV40	Q3V4C2
RefSeq (mRNA)	NM_003761	NM_016794.3
RefSeq (protein)	NP_003752	NP_058074.2
Location (UCSC)	Chr 2: 85.79 – 85.81 Mb	Chr 6: 72.34 – 72.34 Mb
PubMed search	[1]	[2]

Vesicle-associated membrane protein 8 is a protein that in humans is encoded by the VAMP8 gene.

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. It is associated with the perinuclear vesicular structures of the early endocytic compartment. It has been found that VAMP8 interacts specifically with the soluble NSF-attachment protein (alpha-SNAP), most likely through an VAMP8-containing SNARE complex.

Interactions

Vesicle-associated membrane protein 8 has been shown to interact with STX4, SNAP23, STX1A, STX8 and STX7.

Further reading

• Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548. 
• Fasshauer D, Antonin W, Margittai M et al (1999). "Mixed and non-cognate SNARE complexes. Characterization of assembly and biophysical properties". J. Biol. Chem. 274 (22): 15440–6. doi:10.1074/jbc.274.22.15440. PMID 10336434. 
• Kim PK, Hollerbach C, Trimble WS et al (2000). "Identification of the endoplasmic reticulum targeting signal in vesicle-associated membrane proteins". J. Biol. Chem. 274 (52): 36876–82. doi:10.1074/jbc.274.52.36876. PMID 10601239. 
• Paumet F, Le Mao J, Martin S et al (2000). "Soluble NSF attachment protein receptors (SNAREs) in RBL-2H3 mast cells: functional role of syntaxin 4 in exocytosis and identification of a vesicle-associated membrane protein 8-containing secretory compartment". J. Immunol. 164 (11): 5850–7. PMID 10820264. 
• Antonin W, Holroyd C, Tikkanen R et al (2000). "The R-SNARE Endobrevin/VAMP-8 Mediates Homotypic Fusion of Early Endosomes and Late Endosomes". Mol. Biol. Cell 11 (10): 3289–98. PMC 14992. PMID 11029036. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=14992. 
• Antonin W, Holroyd C, Fasshauer D et al (2001). "A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function". EMBO J. 19 (23): 6453–64. doi:10.1093/emboj/19.23.6453. PMC 305878. PMID 11101518. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=305878. 
• Nagamatsu S, Nakamichi Y, Watanabe T et al (2001). "Localization of cellubrevin-related peptide, endobrevin, in the early endosome in pancreatic beta cells and its physiological function in exo-endocytosis of secretory granules". J. Cell. Sci. 114 (Pt 1): 219–227. PMID 11112705. 
• Wade N, Bryant NJ, Connolly LM et al (2001). "Syntaxin 7 complexes with mouse Vps10p tail interactor 1b, syntaxin 6, vesicle-associated membrane protein (VAMP)8, and VAMP7 in b16 melanoma cells". J. Biol. Chem. 276 (23): 19820–7. doi:10.1074/jbc.M010838200. PMID 11278762. 
• Antonin W, Fasshauer D, Becker S et al (2002). "Crystal structure of the endosomal SNARE complex reveals common structural principles of all SNAREs". Nat. Struct. Biol. 9 (2): 107–11. doi:10.1038/nsb746. PMID 11786915. 
• Polgár J, Chung SH, Reed GL (2002). "Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion". Blood 100 (3): 1081–3. doi:10.1182/blood.V100.3.1081. PMID 12130530. 
• Strausberg RL, Feingold EA, Grouse LH et al (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241. 
• Imai A, Nashida T, Yoshie S, Shimomura H (2003). "Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE complexes on the apical plasma membrane". Arch. Oral Biol. 48 (8): 597–604. doi:10.1016/S0003-9969(03)00116-X. PMID 12828989. 
• Gerhard DS, Wagner L, Feingold EA et al (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928. 
• Hillier LW, Graves TA, Fulton RS et al (2005). "Generation and annotation of the DNA sequences of human chromosomes 2 and 4". Nature 434 (7034): 724–31. doi:10.1038/nature03466. PMID 15815621. 
• Brinkman JF, Ottenheim CP, de Jong LA et al (2006). "VAMP5 and VAMP8 are most likely not involved in primary open-angle glaucoma". Mol. Vis. 11: 582–6. PMID 16110299. 
• Oishi Y, Arakawa T, Tanimura A et al (2007). "Role of VAMP-2, VAMP-7, and VAMP-8 in constitutive exocytosis from HSY cells". Histochem. Cell Biol. 125 (3): 273–81. doi:10.1007/s00418-005-0068-y. PMID 16195891. 
• Shiffman D, Rowland CM, Louie JZ et al (2006). "Gene variants of VAMP8 and HNRPUL1 are associated with early-onset myocardial infarction". Arterioscler. Thromb. Vasc. Biol. 26 (7): 1613–8. doi:10.1161/01.ATV.0000226543.77214.e4. PMID 16690874.