Vesicular acetylcholine transporter

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Solute carrier family 18 (vesicular acetylcholine transporter), member 3
Identifiers
Symbols SLC18A3 ; VACHT
External IDs OMIM600336 HomoloGene11022 GeneCards: SLC18A3 Gene
Orthologs
Species Human Mouse
Entrez 6572 20508
Ensembl ENSG00000187714 n/a
UniProt Q16572 n/a
RefSeq (mRNA) NM_003055 NM_021712
RefSeq (protein) NP_003046 NP_068358
Location (UCSC) Chr 10:
50.82 – 50.82 Mb
n/a
PubMed search [1] [2]

The Vesicular acetylcholine transporter (VAChT) is a neurotransmitter transporter which is responsible for loading acetylcholine (ACh) into secretory organelles in neurons making acetylcholine available for secretion.[1][2] It is encoded by Solute carrier family 18, member 3 (SLC18A3) gene. VAChT is able to transport ACh into vesicles by relying on an exchange between protons that were previously pumped into the vesicle diffusing out. ACh molecules are then carried into the cell by the action of exiting protons. [3] Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.

VACh uptake inhibitors[edit]

Radiolabeled compounds[edit]

PET imaging of the VAChT may provide insights into early diagnosis of Alzheimer's disease.

  • (−)-trans-2-Hydroxy-3-(4-(4-[18F]fluorobenzoyl)piperidino)tetralin; racemate: Ki = 2.70 nM for VAChT, 191 nM for σ1, and 251 nM for σ2[4]

References[edit]

  1. ^ Erickson JD, Varoqui H (2000). "Molecular analysis of vesicular amine transporter function and targeting to secretory organelles". FASEB J. 14 (15): 2450–8. doi:10.1096/fj.00-0206rev. PMID 11099462. 
  2. ^ Weihe E, Tao-Cheng JH, Schäfer MK, Erickson JD, Eiden LE (1996). "Visualization of the vesicular acetylcholine transporter in cholinergic nerve terminals and its targeting to a specific population of small synaptic vesicles". Proc. Natl. Acad. Sci. U.S.A. 93 (8): 3547–52. doi:10.1073/pnas.93.8.3547. PMC 39647. PMID 8622973. 
  3. ^ Waymire, Jack. "Storage of ACh". University of Texas Medical Center. 
  4. ^ Tu Z, Efange SM, Xu J, et al (February 2009). "Synthesis and in Vitro and in Vivo Evaluation of (18)F-Labeled Positron Emission Tomography (PET) Ligands for Imaging the Vesicular Acetylcholine Transporter". J. Med. Chem. 52 (5): 1358–69. doi:10.1021/jm8012344. PMC 2765529. PMID 19203271. 

Further Reading[edit]

  • Lau, J. K.; Brown, K. C.; Thornhill, B. A.; Crabtree, C. M.; Dom, A. M.; Witte, T. R.; Hardman, W. E.; McNees, C. A.; Stover, C. A.; Carpenter, A. B.; Luo, H; Chen, Y. C.; Shiflett, B. S.; Dasgupta, P (2013). "Inhibition of cholinergic signaling causes apoptosis in human bronchioalveolar carcinoma". Cancer Research 73 (4): 1328–39. doi:10.1158/0008-5472.CAN-12-3190. PMID 23222296.  edit
  • Varoqui, H; Erickson, J. D. (1996). "Active transport of acetylcholine by the human vesicular acetylcholine transporter". The Journal of Biological Chemistry 271 (44): 27229–32. doi:10.1074/jbc.271.44.27229. PMID 8910293.  edit
  • Harold, D; Peirce, T; Moskvina, V; Myers, A; Jones, S; Hollingworth, P; Moore, P; Lovestone, S; Powell, J; Foy, C; Archer, N; Walter, S; Edmonson, A; McIlroy, S; Craig, D; Passmore, P. A.; Goate, A; Hardy, J; O'Donovan, M; Williams, J; Liddell, M; Owen, M. J.; Jones, L (2003). "Sequence variation in the CHAT locus shows no association with late-onset Alzheimer's disease". Human Genetics 113 (3): 258–67. doi:10.1007/s00439-003-0960-2. PMID 12759818.  edit
  • Morgan, A. R.; Turic, D; Jehu, L; Hamilton, G; Hollingworth, P; Moskvina, V; Jones, L; Lovestone, S; Brayne, C; Rubinsztein, D. C.; Lawlor, B; Gill, M; O'Donovan, M. C.; Owen, M. J.; Williams, J (2007). "Association studies of 23 positional/functional candidate genes on chromosome 10 in late-onset Alzheimer's disease". American Journal of Medical Genetics Part B: Neuropsychiatric Genetics 144B (6): 762–70. doi:10.1002/ajmg.b.30509. PMID 17373700.  edit
  • Schütz, B; Chen, L; Schäfer, M. K.; Weihe, E; Eiden, L. E. (2000). "Somatomotor neuron-specific expression of the human cholinergic gene locus in transgenic mice". Neuroscience 96 (4): 707–22. doi:10.1016/s0306-4522(99)00587-4. PMID 10727789.  edit
  • Tayebati, S. K.; El-Assouad, D; Ricci, A; Amenta, F (2002). "Immunochemical and immunocytochemical characterization of cholinergic markers in human peripheral blood lymphocytes". Journal of neuroimmunology 132 (1-2): 147–55. PMID 12417445.  edit
  • Chireux, M. A.; Le Van Thai, A; Weber, M. J. (1995). "Human choline acetyltransferase gene: Localization of alternative first exons". Journal of Neuroscience Research 40 (4): 427–38. doi:10.1002/jnr.490400402. PMID 7616604.  edit

External links[edit]