Vesicular stomatitis virus
|Vesicular stomatitis Indiana virus|
|TEM micrograph of VSV virions.|
|Group:||Group V ((-)ssRNA)|
|Vesicular stomatitis Indiana virus
Vesicular stomatitis Indiana virus (VSIV) (often still referred to as VSV) is a virus in the family Rhabdoviridae; the well-known rabies virus belongs to the same family. VSIV can infect insects, cattle, horses and pigs. It has particular importance to farmers in certain regions of the world where it can infect cattle. This is because its clinical presentation is identical to the very important foot and mouth disease virus.
The virus is zoonotic and leads to a flu-like illness in infected humans.
Vesicular stomatitis Indiana virus (VSIV) is the prototypic member of the genus Vesiculovirus of the family Rhabdoviridae. The genome of the virus is a single molecule of negative-sense RNA that encodes five major proteins: G protein (G), large protein (L), phosphoprotein, matrix protein (M) and nucleoprotein.
The VSIV G protein enables viral entry. It mediates viral attachment to the host cell, where it is endocytosed. It then mediates fusion of the viral envelope with the endosomal membrane. VSIV enters the cell through partially clathrin-coated vesicles; virus-containing vesicles contain more clathrin and clathrin adaptor than conventional vesicles. Virus-containing vesicles recruit components of the actin machinery for their interaction, thus inducing its own uptake.
The VSIV L protein is encoded by half the genome, and combines with the phosphoprotein to catalyze replication of the mRNA.
The VSIV M protein is encoded by an mRNA that is 831 nucleotides long and translates to a 229 amino acid-protein. The predicted M protein sequence does not contain any long hydrophobic or nonpolar domains that might promote membrane association. The protein is rich in basic amino acids and contains a highly basic amino terminal domain.
After infection, the VSIV G gene is expressed and is commonly studied as a model for N-linked glycosylation in the endoplasmic reticulum (ER). It is translated into the rough ER where the Glc3-Man9-GlcNac2 oligosaccharide is added by a dolichol-containing protein, to an NXS motif on VSIV G. Sugars are removed gradually as the protein travels to the Golgi apparatus, and it becomes resistant to endoglycosidase H.
VSIV G does not follow the same path as most vesicles because transport of the G protein from the ER to the plasma membrane is interrupted by incubation at 15°C. Under this condition, the molecules accumulate in both the ER and a subcellular vesicle fraction of low density called the lipid-rich vesicle fraction. The material in the lipid-rich vesicle fraction appears to be a post-ER intermediate in the transport process to the plasma membrane (PM). When synthesized in polarized epithelial cells, the envelope glycoprotein VSV G is targeted to the basolateral PM. VSVG is also a common coat protein for lentiviral vector expression systems used to introduce genetic material into in vitro systems or animal models, mainly because of its extremely broad tropism.
Clinical signs and diagnosis 
The main sign in animals is oral disease appearing as mucosal vesicles and ulcers in the mouth, but also on the udder and around the coronary band. Animals may show systemic signs such as anorexia, lethargy and pyrexia. Disease usually resolves within two weeks, and animals usually recover completely.
Treatment and control 
No specific treatment is available, but some animals may require supportive fluids or antibiotics for secondary infections.
Medical applications 
Oncolytic therapy 
VSIV has oncolytic activity, leading to preferential killing of infected tumor cells. Recently, attenuated VSIV with a mutation in its M protein has been found to have oncolytic properties. Research is ongoing, and has shown VSIV to reduce tumor size and spread in melanoma, lung cancer, colon cancer and certain brain tumors in laboratory models of cancer.
Other applications 
The VSIV G protein is commonly used in biomedical research to pseudotype retroviral and lentiviral vectors, conveying the ability to transduce a broad range of mammalian cell types with genes of interest.
The VSIV G protein has also been used in cytological studies of trafficking in the endomembrane system. Immunoelectron microscopy suggests that VSIV G protein moves from cis to trans Golgi bodies without being transported between them in vesicles, supporting the cisternal maturation model of Golgi trafficking.
See also 
- Vesicular Stomatitis Virus reviewed and published by WikiVet, accessed 12 October 2011.
- Norkin L.C. (2010.) Virology: Molecular Biology and Pathogenesis American Society for Microbiology, Canada.
- Alberts et al., 2002. Molecular biology of the cell, 4th edition.
- Bell JC, Stojdl D; Lichty; Knowles; Marius; Atkins; Sonenberg (2000). "Exploiting tumor-specific defects in the interferon pathway with a previously unknown oncolytic virus". Nature Medicine 6 (7): 782–789. doi:10.1038/77558. PMID 10888934. Retrieved 2009-04-23.
- Özduman K, Wollman G; Piepmeier; van den Pol (2008). "Systemic Vesicular Stomatitis Virus Selectively Destroys Multifocal Glioma and Metastatic Carcinoma in Brain". The Journal of Neuroscience 28 (8): 1882–1893. doi:10.1523/JNEUROSCI.4905-07.2008. PMID 18287505. Retrieved 2010-05-30.
- Cronin J, Zhang XY, Reiser J (2005). "Altering the tropism of lentiviral vectors through pseudotyping". Curr Gene Ther 5 (4): 387–98. PMID 16101513.
- Mironov AA, Beznoussenko GV, Nicoziani P, Martella O, Trucco A, Kweon H, Giandomenico DD, Polishchuk RS, Fusella A, Lupetti P, Berger EG, Geerts WJC, Koster AJ, Burger KNJ, Luini A (2001). "Small cargo proteins and large aggregates can traverse the Golgi by a common mechanism without leaving the lumen of cisternae". JCB 155 (7): 1225–38. PMID 11756473.