Water blue

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Water blue
Water blue2.png
CAS number 28631-66-5 YesY
Jmol-3D images Image 1
Molecular formula C32H25N3O9S3Na2
Molar mass 737.72374 g/mol
Except where noted otherwise, data are given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
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Infobox references

Water blue, also known as aniline blue, Acid blue 22, Soluble Blue 3M, Marine Blue V, or C.I. 42755, is a chemical compound used as a stain in histology. Water blue stains collagen blue in tissue sections. It is soluble in water and slightly soluble in ethanol.

Water blue is also available in mixture with methyl blue, under name Aniline Blue WS, Aniline blue, China blue, or Soluble blue.

It can be used in the Mallory's connective tissue stain and Gömöri trichrome stain. It is used in differential staining.[1]


  1. ^ Nowicki, Marcin et al. (15 May 2013), A simple dual stain for detailed investigations of plant-fungal pathogen interactions, Vegetable Crops Research bulleting, InHort & Versita, doi:10.2478/v10032-012-0016-z, retrieved 2013-05-24