RhoU (or Wrch1 or Chp2) is a small (~21 kDa) signaling G protein (more specifically a GTPase), and is a member of the Rho family of GTPases. Wrch1 was identified in 2001 as encoded by a non-canonical Wnt induced gene. RhoU/Wrch delineates with [1 ] RhoV/Chp a Rho subclass related to Rac and Cdc42, which emerged in early multicellular organisms during evolution. [2 ]
Model organisms [ edit ]
Model organisms have been used in the study of RhoU function. A conditional knockout mouse line, called Rhou tm1a(KOMP)Wtsi [7 ] was generated as part of the [8 ] International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists — at the Wellcome Trust Sanger Institute. [9 ] [10 ] [11 ]
Male and female animals underwent a standardized
phenotypic screen to determine the effects of deletion. [5 ] Twenty three tests were carried out on [12 ] mutant mice, but no significant abnormalities were observed. [5 ]
References [ edit ]
^ Tao W, Pennica D, Xu L, Kalejta RF and Levine AJ. (2001). "Wrch-1, a novel member of the Rho gene family that is regulated by Wnt-1". Genes Dev 15 (14): 1796–807. doi: 10.1101/gad.894301. ISSN 0890-9369. PMC 312736. PMID 11459829.
^ Boureux A, Vignal E, Faure S, Fort P. (2007). "Evolution of the Rho family of ras-like GTPases in eukaryotes". Mol Biol Evol 24 (1): 203–16. doi: 10.1128/JB.00142-07. ISSN 0021-9193. PMC 1913362. PMID 17449622.
^ ". Wellcome Trust Sanger Institute. Salmonella infection data for Rhou"
^ ". Wellcome Trust Sanger Institute. Citrobacter infection data for Rhou"
^ a b c Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica 88 (S248). doi: 10.1111/j.1755-3768.2010.4142.x.
^ Mouse Resources Portal, Wellcome Trust Sanger Institute.
^ "International Knockout Mouse Consortium".
^ "Mouse Genome Informatics".
^ Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–342. doi: 10.1038/nature10163. PMC 3572410. PMID 21677750.
^ Dolgin E (June 2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi: 10.1038/474262a. PMID 21677718.
^ Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell 128 (1): 9–13. doi: 10.1016/j.cell.2006.12.018. PMID 17218247.
^ van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism.". Genome Biol 12 (6): 224. doi: 10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.