Yakky Doodle (voiced by Jimmy Weldon to sound similar to Donald Duck) is an anthropomorphic yellow duckling with green wings who lives with his best friend Chopper the bulldog. Yakky is always one to run into danger when it is most expected. This usually comes in the form of the show's main antagonist Fibber Fox or secondary villain Alfy Gator. Chopper defends his "Little Buddy" fiercely, and is always ready to pound Fibber or any other enemy into oblivion if necessary. Yakky's tendency to put himself in great danger never draws a rebuke from Chopper, who presumably enjoys his protective role. One of Yakky's repeated line is "Are you my mama?" One of Yakky's favorite songs he loved to sing, in a few episodes, was "Ta-ra-ra Boom-de-ay".
Besides Yakky, the main characters in his cartoons are:
Chopper (voiced by Vance Colvig impersonating Wallace Beery): A bulldog who always protects Yakky from harm. If he thinks that the only way to do this is to send Yakky away, he's willing to pretend he is not the duckling's friend anymore; otherwise, he's the best friend Yakky has. Although he acts tough, in some ways he can be just as childlike as Yakky, such as when he wished he could fly like a bird. Chopper's repeated line is "Now, ain't that cute", as well as saying "Now close your eyes, Yakky. You shouldn't oughta see what I'm going to do to this fox/cat" before beating up the Fibber or the cat. Another repeated quote is when Chopper feels guilty of having to turn Yakky away, and hopes that he didn't "hurt his feelings".
Fibber Fox (voiced by Daws Butler impersonating Shelley Berman): A fox is the main antagonist who first tried to use Yakky as a "bargaining chip" to get the chickens from a henhouse that Chopper was guarding; since then, he has been fixated on catching and eating Yakky, at one point saying that he now hates chicken and wants duck instead. He shows cunning in his plans, most of which are intended to get Chopper out of the way so he can have a clear run at Yakky. For all his predatory behavior, however, he may not be able to eat Yakky. In one episode, he finds that he just can't bring himself to cook or otherwise harm the little duckling, or even let another fox eat him.
The Cat (also voiced by Daws Butler impersonating Shelley Berman): An unnamed cat who is an indirect kin to Fibber Fox also had a similar objective to eat Yakky. However, he often gets punished by Chopper or by the mistress of the house.
Alfy Gator (voiced by Daws Butler impersonating Alfred Hitchcock): A blue alligator is the secondary antagonist who tries to capture Yakky because his gourmet guidebook recommends roast duck. He tends to be verbose while setting his traps, probably as an imitation of Hitchcock's introductions on the TV show Alfred Hitchcock Presents. Also taken from that show is Hitchcock's silhouette & outline routine, which Alfy imitates in most of the cartoons he's in. One time he found himself competing with Fibber to catch Yakky, but the two predators eventually agreed to work together to capture the duck (the cartoon ended during the final chase, but presumably Yakky got away).