Zona pellucida sperm-binding protein 3, also known as zona pellucida glycoprotein 3 (Zp-3) or the sperm receptor, is a protein that in humans is encoded by the ZP3gene. ZP3 is the receptor in the zona pellucida which binds sperm at the beginning of fertilization.
The zona pellucida (ZP) is a specialized extracellular matrix that surrounds the oocyte and early embryo. It is composed of three or four glycoproteins (ZP1-4) with various functions during oogenesis, fertilization and preimplantation development. The protein encoded by this gene is a major structural component of the ZP and functions in primary binding and simulation of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site (CFCS), a polymerization-blocking external hydrophobic patch (EHP), and a C-terminal transmembrane domain. Cleavage at the CFCS separates the mature protein from the EHP, allowing it to incorporate into nascent ZP filaments. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene.
^van Duin M, Polman JE, Verkoelen CC, Bunschoten H, Meyerink JH, Olijve W, Aitken RJ (December 1992). "Cloning and characterization of the human sperm receptor ligand ZP3: evidence for a second polymorphic allele with a different frequency in the Caucasian and Japanese populations". Genomics14 (4): 1064–70. doi:10.1016/S0888-7543(05)80130-2. PMID1478648.
Choudhury S, Ganguly A, Chakrabarti K, et al. (2009). "DNA vaccine encoding chimeric protein encompassing epitopes of human ZP3 and ZP4: immunogenicity and characterization of antibodies". J. Reprod. Immunol.79 (2): 137–47. doi:10.1016/j.jri.2008.09.002. PMID19004505.
Chiu PC, Wong BS, Chung MK, et al. (2008). "Effects of native human zona pellucida glycoproteins 3 and 4 on acrosome reaction and zona pellucida binding of human spermatozoa". Biol. Reprod.79 (5): 869–77. doi:10.1095/biolreprod.108.069344. PMID18667750.
Gook DA, Edgar DH, Borg J, Martic M (2008). "Detection of zona pellucida proteins during human folliculogenesis". Hum. Reprod.23 (2): 394–402. doi:10.1093/humrep/dem373. PMID18033806.
Törmälä RM, Jääskeläinen M, Lakkakorpi J, et al. (2008). "Zona pellucida components are present in human fetal ovary before follicle formation". Mol. Cell. Endocrinol.289 (1-2): 10–5. doi:10.1016/j.mce.2008.01.029. PMID18502569.
Ni Y, Li K, Xu W, et al. (2007). "Acrosome reaction induced by recombinant human zona pellucida 3 peptides rhuZP3a22 approximately 176 and rhuZP3b177 approximately 348 and their mechanism". J. Androl.28 (3): 381–8. doi:10.2164/jandrol.106.001289. PMID17192598.
Furlong LI, Harris JD, Vazquez-Levin MH (2005). "Binding of recombinant human proacrosin/acrosin to zona pellucida (ZP) glycoproteins. I. Studies with recombinant human ZPA, ZPB, and ZPC". Fertil. Steril.83 (6): 1780–90. doi:10.1016/j.fertnstert.2004.12.042. PMID15950651.
Zhao M, Boja ES, Hoodbhoy T, et al. (2004). "Mass spectrometry analysis of recombinant human ZP3 expressed in glycosylation-deficient CHO cells". Biochemistry43 (38): 12090–104. doi:10.1021/bi048958k. PMID15379548.
Jovine L, Qi H, Williams Z, Litscher E, Wassarman PM (2002). "The ZP domain is a conserved module for polymerization of extracellular proteins". Nat Cell Biol.4 (6): 457–61. doi:10.1038/ncb802. PMID12021773.