Zombo District is bordered by Arua District to the north, Nebbi District to the east, and the Democratic Republic of the Congo to the south and west. Zombo, where the district headquarters are located is approximately 70 kilometres (43 mi), by road, south of Arua, the largest city in the sub-region. This location lies approximately 382 kilometres (237 mi), by road, northwest of Kampala, the capital of Uganda, and the largest city in that country. The coordinates of the district are:02 30N, 30 54E.
In 1991, the national population census estimated the district population at about 131,300. In 2002, the national census estimated the population of the district at about 169,000. In 2012, the population of Zombo District was estimated at approximately 219,800.