1627 in science
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- Johannes Kepler's Rudolphine Tables are published. These include predictions of Transits of Mercury and Venus.
- January – The Dutch ship 't Gulden Zeepaert, skippered by François Thijssen, sails along the south coast of Australia.
- Adriaan van den Spiegel's De humani corporis fabrica is published posthumously in Venice with illustrations by Giulio Casserio.
- Gaspare Aselli's De lactibus sive lacteis venis is published posthumously in Milan.
- January 25 – Robert Boyle, Anglo-Irish chemist (died 1691)
- November 29 – John Ray, English naturalist (died 1705)