1B may refer to:
Science and technology
- Alpha-1B adrenergic receptor
- Arginine vasopressin receptor 1B
- Interferon beta-1b
- Melatonin receptor 1B
- The ASCII hexadecimal code for the escape character
- Axle arrangement on steam locomotives corresponding to a 2-4-0 wheel arrangement
- GCR Class 1B, a class of British 2-6-4T steam locomotive
- National Highway 1B (India)
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