2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

From Wikipedia, the free encyclopedia
Jump to: navigation, search
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Identifiers
EC number 4.6.1.12
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / EGO

2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (MEcPP synthase, IspF, EC 4.6.1.12) is a zinc-dependent enzyme which participates in the MEP pathway (non-mevalonate pathway) of isoprenoid precursor biosynthesis. It catalyzes the chemical reaction:

4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP

Hence, this enzyme has one substrate, 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-MEP), and two products, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MEcPP) and CMP.[1]

The enzyme is considered a phosphorus-oxygen lyase. The systematic name of this enzyme class is 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol CMP-lyase (cyclizing; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate-forming). Other names in common use include IspF, YgbB and MEcPP synthase.

Structural studies[edit]

As of late 2007, 20 structures have been solved for this class of enzymes, with PDB accession codes 1GX1, 1H47, 1H48, 1IV1, 1IV2, 1IV3, 1IV4, 1T0A, 1U3L, 1U3P, 1U40, 1U43, 1VH8, 1VHA, 1W55, 1W57, 1YQN, 2AMT, 2GZL, and 2PMP.

References[edit]

  1. ^ Herz S, Wungsintaweekul J, Schuhr CA, Hecht S, Lüttgen H, Sagner S, Fellermeier M, Eisenreich W, Zenk MH, Bacher A, Rohdich F (2000). "Biosynthesis of terpenoids: YgbB protein converts 4-diphosphocytidyl-2C-methyl-d-erythritol 2-phosphate to 2C-methyl-d-erythritol 2,4-cyclodiphosphate". Proc. Natl. Acad. Sci. USA. 97 (6): 2486–90. doi:10.1073/pnas.040554697. PMC 15955Freely accessible. PMID 10694574.