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2S may refer to:
- 2S, key signature of two sharps
- a series of Toyota S engines
- Birdman Chinook 2S, ultralight aircraft
- 2s electron in an atomic orbital
- 2szTV, a television channel in Senegal
- 2S, a protein-folding intermediate with two disulphide bonds
- Two-spirit, a term used by some indigenous North Americans to describe certain people in their communities who fulfill a traditional third-gender (or other gender-variant) ceremonial role in their cultures
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