The teams have been divided into two zones. East has four teams while West has three after AFC Cup champions Kuwait SC's failure to fulfil the criteria set by AFC to compete in the play-offs. The draw for the qualifying play-off was held on 7 December 2009 in Kuala Lumpur, Malaysia. All losers from the qualifying play-off will enter the 2010 AFC Cup.
Each club plays double round-robin (home and away) against fellow three group members, a total of 6 matches each. Clubs receive 3 points for a win, 1 point for a tie, 0 points for a loss. The clubs are ranked according to points and tie breakers are in following order:
Greater number of points obtained in the group matches between the teams concerned;
Goal difference resulting from the group matches between the teams concerned; (Away goals do not apply)
Greater number of goals scored in the group matches between the teams concerned; (Away goals do not apply)
Goal difference in all the group matches;
Greater number of goals scored in all the group matches;
Kicks from the penalty mark if only two teams are involved and they are both on the field of play;
Fewer score calculated according to the number of yellow and red cards received in the group matches; (1 point for each yellow card, 3 points for each red card as a consequence of two yellow cards, 3 points for each direct red card, 4 points for each yellow card followed by a direct red card)
Drawing of lots.
Winners and runners-up of each group will qualify for the next round.
The draw for the remaining rounds was held in Kuala Lumpur, Malaysia on 25 May 2010. Because of the country protection rule, if there are two clubs from the same country, they will not face each other in the quarter-finals. Therefore, the two clubs from Saudi Arabia may not be drawn with each other in the quarter-finals. However, the same rule does not apply if there are more than two clubs from the country. Therefore, the four clubs from the Korea Republic may be drawn with each other in the quarter-finals.
The first legs were played on 15 September, and the second legs were played on 22 September 2010.