2LO was the second radio station to regularly broadcast in the United Kingdom (the first was 2MT). It began broadcasting on 11 May 1922, for one hour a day from the seventh floor of Marconi House in London's Strand. This building, opposite Somerset House, was demolished in 2006, apart from the listed façade, which will be incorporated into a new hotel complex. A first hand account of a broadcast from 2LO is given in The Spell of London by Henry Vollam Morton.
Initially the power was 100 watts on 350 metres (857 kHz). 2LO was allowed to transmit for seven minutes, after which the 'operator' had to listen on the wavelength for three minutes for possible instructions to close down. On 14 November 1922 the station was transferred to the new British Broadcasting Company which in 1923 took up the nearby Savoy Hill for its broadcasting studios. In 1927 the company became the British Broadcasting Corporation. On 9 March 1930 2LO was replaced by the BBC Regional Programme and the BBC National Programme. The letters LO continued to be used internally as a designation in the BBC for technical operations in the London area (for example the numbering of all recordings made in London contained LO). The code LO was changed to LN in the early 1970s.
The 2LO transmitter now belongs to the Science Museum, having been donated by Crown Castle International on 7 November 2002.  It is now on show in the Information Age gallery on the second floor of the museum.
The 'LO' part of 2LO's callsign was adopted in 1924 by the metropolitan radio station in Melbourne which, since 1932, has been a part of the Australian Broadcasting Corporation. The station, 3LO, still has this callsign allocated to it but, since 2000 it has used different on-air names, firstly 774 ABC Melbourne and since 2017, Radio Melbourne. The amateur radio callsign G2LO is currently held by the staff association at Arqiva, formerly Crown Castle International, formerly the domestic part of BBC Transmitter Department.
- Marconi House, Strand / Aldwych, London The Music Hall and Theatre Site
- Gift to nation marks BBC's 80th anniversary BBC Press Office, 3 November 2002
- Radio Melbourne was formerly the slogan for commercial station 3AW. Both 3LO and 3AW are considered rivals for the same audience.
- QRZ.com Callsign Database
- H.V, Morton. 1926, 18th Edition 1948, 'The spell of London', Methuen & Co Ltd, London.
- The Science Museum: London Calling
- History of Marconi House where 2LO first broadcast
- Audio of 2LO station identification