4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase

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4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase
EC number
IntEnz IntEnz view
ExPASy NiceZyme view
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase (EC, tesD (gene), hsaD (gene)) is an enzyme with systematic name 4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase ( (2Z,4Z)-2-hydroxyhexa-2,4-dienoate-forming).[1][2][3][4] This enzyme catalyses the following chemical reaction

(1E,2Z)-3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate + H2O 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + (2Z,4Z)-2-hydroxyhexa-2,4-dienoate

The enzyme is involved in the bacterial degradation of the steroid ring structure.


  1. ^ Horinouchi, M.; Hayashi, T.; Koshino, H.; Kurita, T.; Kudo, T. (2005). "Identification of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid, 4-hydroxy-2-oxohexanoic acid, and 2-hydroxyhexa-2,4-dienoic acid and related enzymes involved in testosterone degradation in Comamonas testosteroni TA441". Appl. Environ. Microbiol. 71: 5275–5281. doi:10.1128/aem.71.9.5275-5281.2005. PMC 1214608. PMID 16151114.
  2. ^ Van der Geize, R.; Yam, K.; Heuser, T.; Wilbrink, M.H.; Hara, H.; Anderton, M.C.; Sim, E.; Dijkhuizen, L.; Davies, J.E.; Mohn, W.W.; Eltis, L.D. (2007). "A gene cluster encoding cholesterol catabolism in a soil actinomycete provides insight into Mycobacterium tuberculosis survival in macrophages". Proc. Natl. Acad. Sci. USA. 104: 1947–1952. doi:10.1073/pnas.0605728104. PMC 1794314. PMID 17264217.
  3. ^ Lack, N.; Lowe, E.D.; Liu, J.; Eltis, L.D.; Noble, M.E.; Sim, E.; Westwood, I.M. (2008). "Structure of HsaD, a steroid-degrading hydrolase, from Mycobacterium tuberculosis". Acta Crystallographica Section F. 64: 2–7. doi:10.1107/s1744309107065931. PMC 2373992. PMID 18097091.
  4. ^ Lack, N.A.; Yam, K.C.; Lowe, E.D.; Horsman, G.P.; Owen, R.L.; Sim, E.; Eltis, L.D. (2010). "Characterization of a carbon-carbon hydrolase from Mycobacterium tuberculosis involved in cholesterol metabolism". J. Biol. Chem. 285: 434–443. doi:10.1074/jbc.m109.058081. PMC 2804191. PMID 19875455.

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