4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase

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4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase
Identifiers
EC number 3.7.1.17
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase (EC 3.7.1.17, tesD (gene), hsaD (gene)) is an enzyme with systematic name 4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase ( (2Z,4Z)-2-hydroxyhexa-2,4-dienoate-forming).[1][2][3][4] This enzyme catalyses the following chemical reaction

(1E,2Z)-3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate + H2O 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + (2Z,4Z)-2-hydroxyhexa-2,4-dienoate

The enzyme is involved in the bacterial degradation of the steroid ring structure.

References[edit]

  1. ^ Horinouchi, M.; Hayashi, T.; Koshino, H.; Kurita, T.; Kudo, T. (2005). "Identification of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid, 4-hydroxy-2-oxohexanoic acid, and 2-hydroxyhexa-2,4-dienoic acid and related enzymes involved in testosterone degradation in Comamonas testosteroni TA441". Appl. Environ. Microbiol. 71: 5275–5281. PMC 1214608Freely accessible. PMID 16151114. doi:10.1128/aem.71.9.5275-5281.2005. 
  2. ^ Van der Geize, R.; Yam, K.; Heuser, T.; Wilbrink, M.H.; Hara, H.; Anderton, M.C.; Sim, E.; Dijkhuizen, L.; Davies, J.E.; Mohn, W.W.; Eltis, L.D. (2007). "A gene cluster encoding cholesterol catabolism in a soil actinomycete provides insight into Mycobacterium tuberculosis survival in macrophages". Proc. Natl. Acad. Sci. USA. 104: 1947–1952. PMC 1794314Freely accessible. PMID 17264217. doi:10.1073/pnas.0605728104. 
  3. ^ Lack, N.; Lowe, E.D.; Liu, J.; Eltis, L.D.; Noble, M.E.; Sim, E.; Westwood, I.M. (2008). "Structure of HsaD, a steroid-degrading hydrolase, from Mycobacterium tuberculosis". Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 64: 2–7. PMC 2373992Freely accessible. PMID 18097091. doi:10.1107/s1744309107065931. 
  4. ^ Lack, N.A.; Yam, K.C.; Lowe, E.D.; Horsman, G.P.; Owen, R.L.; Sim, E.; Eltis, L.D. (2010). "Characterization of a carbon-carbon hydrolase from Mycobacterium tuberculosis involved in cholesterol metabolism". J. Biol. Chem. 285: 434–443. PMC 2804191Freely accessible. PMID 19875455. doi:10.1074/jbc.m109.058081. 

External links[edit]